Nottingham University Research and Treatment Unit in Reproduction (NURTURE), School of Human Development, Queen's Medical Centre, Nottingham University Hospitals NHS Trust, University of Nottingham, Nottingham, UK.
Ultrasound Obstet Gynecol. 2009 Apr;33(4):477-83. doi: 10.1002/uog.6310.
To assess the reliability of automated measurements of the total antral follicle count (AFC) made using Sono-Automatic Volume Count (SonoAVC), and to compare these to two-dimensional (2D) and manual three-dimensional (3D) techniques.
Fifty-five subjects aged under 40 years who had 3D transvaginal ultrasound examination in the early follicular phase of their menstrual cycle were prospectively recruited. 3D datasets were acquired and subsequently analyzed. The total AFC (2-10 mm antral follicles) was calculated by two observers using three independent methods: 2D real-time equivalent (2D-RTE), 3D manual multiplanar view (3D-MPV), and SonoAVC. For measurements made using SonoAVC, the initial automated count (sAVC-AA) was recorded and postprocessing (sAVC-PP) then applied to identify follicles that had been missed or incorrectly included. Intraclass correlation and limits of agreement were used to evaluate the methods.
The intra- and interobserver reliability of measurements of total AFC was best with SonoAVC with postprocessing followed by 3D-MPV and 2D-RTE. The initial count calculated by sAVC-AA missed follicles and this was reflected in the significantly lower mean total AFC (6.51 +/- 4.79) than that made after postprocessing techniques (sAVC-PP, 18.42 +/- 10.53, P < 0.001; 3D-MPV, 19.38 +/- 10.85, P < 0.001; and 2D-RTE, 19.26 +/- 10.55, P < 0.001). The mean total AFC became more comparable with postprocessing (sAVC-PP) but still remained significantly lower than counts made with 2D-RTE and 3D-MPV (P < 0.05).
SonoAVC with postprocessing is a reliable method for measuring total AFC. It takes longer to perform, because of the need for postprocessing, and obtains values that are lower than those obtained by the 2D and 3D-MPV techniques. However, the AFC obtained by sAVC-PP is likely to be lower because this method measures and color codes each follicle preventing recounting.
评估 Sono-Automatic Volume Count（SonoAVC）自动测量总窦卵泡计数（AFC）的可靠性，并将其与二维（2D）和手动三维（3D）技术进行比较。
前瞻性招募 55 名年龄在 40 岁以下的受试者，在月经周期的早期卵泡期进行 3D 经阴道超声检查。采集 3D 数据集并进行后续分析。两名观察者使用三种独立的方法计算总 AFC（2-10mm 窦卵泡）：2D 实时等效（2D-RTE）、3D 手动多平面视图（3D-MPV）和 SonoAVC。对于使用 SonoAVC 进行的测量，记录初始自动计数（sAVC-AA），然后应用后处理（sAVC-PP）以识别错过或错误包含的卵泡。使用组内相关系数和一致性界限评估方法。
使用 SonoAVC 进行后处理的总 AFC 测量的观察者内和观察者间可靠性最好，其次是 3D-MPV 和 2D-RTE。sAVC-AA 计算的初始计数遗漏了卵泡，这反映在明显较低的平均总 AFC（6.51±4.79）上，低于后处理技术（sAVC-PP，18.42±10.53，P<0.001；3D-MPV，19.38±10.85，P<0.001；和 2D-RTE，19.26±10.55，P<0.001）。总 AFC 在进行后处理（sAVC-PP）后变得更具可比性，但仍明显低于 2D-RTE 和 3D-MPV 计数（P<0.05）。
使用后处理的 SonoAVC 是一种可靠的测量总 AFC 的方法。由于需要后处理，因此需要更长的时间，并且获得的值低于 2D 和 3D-MPV 技术获得的值。然而，sAVC-PP 获得的 AFC 可能较低，因为该方法测量并对每个卵泡进行颜色编码，从而防止重新计数。
