三维超声和 SonoAVC 自动窦卵泡计数的观察者内和观察者间可靠性。

Intraobserver and interobserver reliability of automated antral follicle counts made using three-dimensional ultrasound and SonoAVC.

机构信息

Nottingham University Research and Treatment Unit in Reproduction (NURTURE), School of Human Development, Queen's Medical Centre, Nottingham University Hospitals NHS Trust, University of Nottingham, Nottingham, UK.

出版信息

Ultrasound Obstet Gynecol. 2009 Apr;33(4):477-83. doi: 10.1002/uog.6310.

DOI:10.1002/uog.6310

PMID:19212944

Abstract

OBJECTIVE

To assess the reliability of automated measurements of the total antral follicle count (AFC) made using Sono-Automatic Volume Count (SonoAVC), and to compare these to two-dimensional (2D) and manual three-dimensional (3D) techniques.

METHODS

Fifty-five subjects aged under 40 years who had 3D transvaginal ultrasound examination in the early follicular phase of their menstrual cycle were prospectively recruited. 3D datasets were acquired and subsequently analyzed. The total AFC (2-10 mm antral follicles) was calculated by two observers using three independent methods: 2D real-time equivalent (2D-RTE), 3D manual multiplanar view (3D-MPV), and SonoAVC. For measurements made using SonoAVC, the initial automated count (sAVC-AA) was recorded and postprocessing (sAVC-PP) then applied to identify follicles that had been missed or incorrectly included. Intraclass correlation and limits of agreement were used to evaluate the methods.

RESULTS

The intra- and interobserver reliability of measurements of total AFC was best with SonoAVC with postprocessing followed by 3D-MPV and 2D-RTE. The initial count calculated by sAVC-AA missed follicles and this was reflected in the significantly lower mean total AFC (6.51 +/- 4.79) than that made after postprocessing techniques (sAVC-PP, 18.42 +/- 10.53, P < 0.001; 3D-MPV, 19.38 +/- 10.85, P < 0.001; and 2D-RTE, 19.26 +/- 10.55, P < 0.001). The mean total AFC became more comparable with postprocessing (sAVC-PP) but still remained significantly lower than counts made with 2D-RTE and 3D-MPV (P < 0.05).

CONCLUSION

SonoAVC with postprocessing is a reliable method for measuring total AFC. It takes longer to perform, because of the need for postprocessing, and obtains values that are lower than those obtained by the 2D and 3D-MPV techniques. However, the AFC obtained by sAVC-PP is likely to be lower because this method measures and color codes each follicle preventing recounting.

摘要

目的

评估 Sono-Automatic Volume Count（SonoAVC）自动测量总窦卵泡计数（AFC）的可靠性，并将其与二维（2D）和手动三维（3D）技术进行比较。

方法

前瞻性招募 55 名年龄在 40 岁以下的受试者，在月经周期的早期卵泡期进行 3D 经阴道超声检查。采集 3D 数据集并进行后续分析。两名观察者使用三种独立的方法计算总 AFC（2-10mm 窦卵泡）：2D 实时等效（2D-RTE）、3D 手动多平面视图（3D-MPV）和 SonoAVC。对于使用 SonoAVC 进行的测量，记录初始自动计数（sAVC-AA），然后应用后处理（sAVC-PP）以识别错过或错误包含的卵泡。使用组内相关系数和一致性界限评估方法。

结果

使用 SonoAVC 进行后处理的总 AFC 测量的观察者内和观察者间可靠性最好，其次是 3D-MPV 和 2D-RTE。sAVC-AA 计算的初始计数遗漏了卵泡，这反映在明显较低的平均总 AFC（6.51±4.79）上，低于后处理技术（sAVC-PP，18.42±10.53，P<0.001；3D-MPV，19.38±10.85，P<0.001；和 2D-RTE，19.26±10.55，P<0.001）。总 AFC 在进行后处理（sAVC-PP）后变得更具可比性，但仍明显低于 2D-RTE 和 3D-MPV 计数（P<0.05）。