Li Xi-He, Yang Xin-Ming, Wu Xiao-Ke
Department of Obstetrics and Gynecology, The First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2008 Nov;28(11):1001-4.
To study the effects of cryptotanshinone on androgen synthesis for the prenatally androgenized male rats.
On days 16-18 of pregnancy, rats were injected s. c. with testosterone propionas continuously for 3 days; male offspring were studied as subject. Serum concentrations of testosterone (T), 17a-hydroxy progesterone (17-OHP), blood glucose, and insulin were measured by radioimmunoassay. Then, the rats were treated with cryptotanshinone by gavage for 14 days, and the levels of serum T, 17-OHP and insulin were detected and the 17a-hydroxylase protein expression in interstitial cell was measured using the method of immunohistochemistry.
There was no difference between the male groups who were prenatally androgenized in serum levels of T, but the 17-OHP, fasting insulin levels and homeostatic model assessment for insulin resistance (HOMA-IR) elevated significantly (P < 0.05). Cryptotanshinone could lower the levels of 17-OHP (P < 0.05) but had no effect on 17a-hydroxylase.
Prenatally androgenized male rats exhibit elevated 17-OHP and diminished insulin sensitivity. Cryptotanshinone could decrease 17-OHP, but has no effect on insulin, indicating it may reduce androgen synthesis.
研究隐丹参酮对产前雄激素化雄性大鼠雄激素合成的影响。
在妊娠第16 - 18天，大鼠皮下连续注射丙酸睾酮3天；以雄性后代作为研究对象。采用放射免疫分析法测定血清睾酮（T）、17α-羟孕酮（17 - OHP）、血糖和胰岛素浓度。然后，大鼠经口灌胃给予隐丹参酮14天，检测血清T、17 - OHP和胰岛素水平，并采用免疫组织化学方法检测间质细胞中17α-羟化酶蛋白表达。
产前雄激素化的雄性组之间血清T水平无差异，但17 - OHP、空腹胰岛素水平和胰岛素抵抗稳态模型评估（HOMA - IR）显著升高（P < 0.05）。隐丹参酮可降低17 - OHP水平（P < 0.05），但对17α-羟化酶无影响。
产前雄激素化的雄性大鼠表现出17 - OHP升高和胰岛素敏感性降低。隐丹参酮可降低17 - OHP，但对胰岛素无影响，表明其可能减少雄激素合成。
