Noroozzadeh M, Ramezani Tehrani F, Sedaghat K, Godini A, Azizi F
Reproductive Endocrinology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Endocrine Physiology Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Endocrinol Invest. 2015 May;38(5):489-95. doi: 10.1007/s40618-014-0198-y. Epub 2014 Dec 21.
In our previous study, we introduced a rat model of polycystic ovary syndrome (PCOS) induced by prenatal exposure to a single dose of testosterone on embryonic day 20. In the current study, we aimed to investigate whether prenatal exposure to a single dose of testosterone could also induce metabolic disturbances, especially insulin resistance in adulthood (100-110 days of age) and also to make it as an appropriate rat model of PCOS (exhibiting both reproductive and metabolic disturbances with minimum morphological disorders in reproductive system) for further studies in PCOS.
Pregnant rats in the experimental group were subcutaneously injected with 5 mg free testosterone on the gestational day 20, while controls received only the solvent. Female offspring of both groups, prenatally androgenized (PNA) rats (PCOS models of rats) and controls were examined.
Body weight measures showed significant increase in the PNA rats compared to controls on days 30, 45, 60 of age and in adulthood (P < 0.05). PNA rats showed insulin resistance compared to controls. Impaired glucose tolerance was not observed in the PNA rats compared to controls. There were no significant differences in lipid profile between the PNA and control rats (P > 0.05).
Our study suggests that metabolic disturbances in PCOS and their severity during adult life probably depend on the particular time and levels of prenatal androgen exposure.
在我们之前的研究中，我们引入了一种多囊卵巢综合征（PCOS）大鼠模型，该模型通过在胚胎第20天产前单次暴露于睾酮诱导产生。在当前研究中，我们旨在调查产前单次暴露于睾酮是否也会诱发代谢紊乱，尤其是成年期（100 - 110日龄）的胰岛素抵抗，并且使其成为一种合适的PCOS大鼠模型（表现出生殖和代谢紊乱，同时生殖系统形态紊乱最小），用于PCOS的进一步研究。
实验组的怀孕大鼠在妊娠第20天皮下注射5毫克游离睾酮，而对照组仅接受溶剂。对两组的雌性后代，即产前雄激素化（PNA）大鼠（大鼠PCOS模型）和对照组进行检查。
体重测量显示，与对照组相比，PNA大鼠在30日龄、45日龄、60日龄及成年期体重显著增加（P < 0.05）。与对照组相比，PNA大鼠表现出胰岛素抵抗。与对照组相比，未在PNA大鼠中观察到糖耐量受损。PNA大鼠和对照大鼠之间的血脂谱无显著差异（P > 0.05）。
我们的研究表明，PCOS中的代谢紊乱及其在成年期的严重程度可能取决于产前雄激素暴露的特定时间和水平。
