Xia Yanqiu, Zhao Pei, Huang Hongli, Xie Yuan, Lu Rong, Dong Li
Department of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Affiliated Hospital of Shanghai University of Traditional Chinese MedicineShanghai 200437, China.
School of Basic Medical Science of Shanghai University of Traditional Chinese MedicineShanghai 201203, China.
Am J Transl Res. 2017 May 15;9(5):2447-2456. eCollection 2017.
Polycystic ovarian syndrome (PCOS), with a prevalence of 5-8%, is the most common endocrine disorder in women of reproductive age. Although cryptotanshinone (CRY) has been shown to effectively reverse reproductive disturbances, it remains poorly understood whether it can be used to treat PCOS. Here we used an established rat model of PCOS. Prepubertal female Sprague-Dawley rats were subcutaneously injected with dehydroepiandrosterone (DHEA) 60 mg/kg/day for 3 weeks. Those in the DHEA group were given normal saline, while the CRY group was given 27 mg/kg by gastrogavage for 3 weeks. DHEA-treated rats had irregular estrous cycles and significantly increased ovarian weight compared to control rats ( < 0.05), and 3 weeks of treatment with CRY in DHEA-treated rats significantly decreased ovarian weight ( < 0.05). CRY decreased the levels of testosterone, estradiol, and luteinizing hormone (LH) as well as the LH/follicle stimulating hormone ratio ( < 0.05). CRY decreased inhibin B and follistatin mRNA and protein expression but upregulated activin A mRNA and protein expression in the ovarian tissue of rats with PCOS ( < 0.05). These results suggest that CRY treatment could reverse reproductive disturbances in rats with PCOS.
多囊卵巢综合征（PCOS）的患病率为5%-8%，是育龄女性中最常见的内分泌紊乱疾病。尽管隐丹参酮（CRY）已被证明能有效逆转生殖功能紊乱，但它是否可用于治疗PCOS仍知之甚少。在此，我们使用了已建立的PCOS大鼠模型。青春期前的雌性斯普拉格-道利大鼠皮下注射60 mg/kg/天的脱氢表雄酮（DHEA），持续3周。DHEA组给予生理盐水，而CRY组通过灌胃给予27 mg/kg，持续3周。与对照大鼠相比，DHEA处理的大鼠发情周期不规律，卵巢重量显著增加（<0.05），在DHEA处理的大鼠中用CRY治疗3周可显著降低卵巢重量（<0.05）。CRY降低了睾酮、雌二醇和黄体生成素（LH）的水平以及LH/卵泡刺激素比值（<0.05）。CRY降低了PCOS大鼠卵巢组织中抑制素B和卵泡抑素的mRNA和蛋白表达，但上调了激活素A的mRNA和蛋白表达（<0.05）。这些结果表明，CRY治疗可逆转PCOS大鼠的生殖功能紊乱。
