用单纳米颗粒探测亲吻-逃离和囊泡再利用的动态控制。
The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles.
作者信息
Zhang Qi, Li Yulong, Tsien Richard W
机构信息
Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA.
出版信息
Science. 2009 Mar 13;323(5920):1448-53. doi: 10.1126/science.1167373. Epub 2009 Feb 12.
Abstract
Vesicular secretion of neurotransmitter is essential for neuronal communication. Kiss-and-run is a mode of membrane fusion and retrieval without the full collapse of the vesicle into the plasma membrane and de novo regeneration. The importance of kiss-and-run during efficient neurotransmission has remained in doubt. We developed an approach for loading individual synaptic vesicles with single quantum dots. Their size and pH-dependent photoluminescence change allowed us to distinguish kiss-and-run from full-collapse fusion and to track single vesicles through multiple rounds of kiss-and-run and reuse, without perturbing vesicle cycling. Kiss-and-run dominated at the beginning of stimulus trains, reflecting the preference of vesicles with high release probability. Its incidence was increased by rapid firing, a response appropriate to shape the kinetics of neurotransmission during a wide range of firing patterns.
摘要
神经递质的囊泡分泌对于神经元通讯至关重要。吻-跑是一种膜融合和回收模式，囊泡不会完全塌陷到质膜中并重新生成。吻-跑在高效神经传递过程中的重要性一直存在疑问。我们开发了一种用单量子点加载单个突触囊泡的方法。它们的大小和pH依赖性光致发光变化使我们能够区分吻-跑和完全塌陷融合，并在不干扰囊泡循环的情况下，通过多轮吻-跑和再利用来追踪单个囊泡。吻-跑在刺激序列开始时占主导地位，反映了具有高释放概率的囊泡的偏好。快速发放增加了其发生率，这是一种适合在广泛发放模式下塑造神经传递动力学的反应。
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The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles.用单纳米颗粒探测亲吻-逃离和囊泡再利用的动态控制。
Science. 2009 Mar 13;323(5920):1448-53. doi: 10.1126/science.1167373. Epub 2009 Feb 12.
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Comment on "The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles".关于《用单纳米颗粒探测亲吻-逃离和囊泡再利用的动态控制》的评论
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