Department of Animal Nutrition, College of Veterinary Science, Sri Venkateswara Veterinary University, Rajendranagar, Hyderabad, 500 030, India.
Vet Res Commun. 2009 Oct;33(7):631-44. doi: 10.1007/s11259-009-9212-9. Epub 2009 Feb 13.
An experiment of 150 days was conducted on 42 male Nellore lambs (28.3 +/- 0.64 kg) to determine the effect of zinc (Zn) supplementation (0,15, 30 and 45 ppm) in diet from inorganic (ZnSO(4)) and organic (Zn proteinate) sources on immune response and antioxidant enzyme activities by allotting them randomly to 7 groups in completely randomized design. The basal diet (BD) contained 29.28 ppm Zn. The humoral immune response assessed at 75 d against B. abortus was higher (P<0.01) with 15 or 30 ppm Zn supplementation from organic source. The dose and source had no effect on titres against chicken RBC antigen. The cell mediated immune response assessed as delayed type hypersensitivity (DTH) response against phytohaemagglutinin-P and in vitro lymphocyte proliferative response against concanavalin A at 150 d was higher (P<0.05) at 15 ppm Zn supplementation compared to BD fed lambs. Supplementation of 45 ppm Zn had no positive effect on immune response. The DTH response and antibody titres against B.abortus were higher (P< 0.05) on Zn proteinate compared to ZnSO(4) at 15 ppm Zn supplementation. The lipid peroxidase activity was lower (P < 0.01), while the RBC superoxide dismutase and catalase activities were higher (P < 0.01) in lambs at 15 ppm Zn supplementation compared to BD diet fed lambs, assessed at 75 d of feeding. Serum globulin concentration and alkaline phosphatase (ALP) activity (75 d of experiment) was higher in Zn supplemented lambs. The ALP activity increased (P < 0.01) with increase in Zn supplementation and being higher when supplementation was from Zn proteinate compared to ZnSO(4). The study indicated that 15 ppm zinc supplementation was required for obtaining higher immune response in lambs when fed a basal diet containing 29.28 ppm Zn and supplementation as Zn proteinate had higher antioxidant enzyme activities and immune response compared to ZnSO(4).
一项为期 150 天的实验在 42 只雄性内罗尔羔羊（28.3±0.64 公斤）身上进行，以确定日粮中不同锌（Zn）添加水平（0、15、30 和 45ppm）对免疫反应和抗氧化酶活性的影响，这些羔羊被随机分配到 7 个完全随机设计的组中。基础日粮（BD）含有 29.28ppm 的锌。在第 75 天，用 B.abortus 进行的体液免疫反应，用有机（Zn 蛋白）来源的 15 或 30ppm Zn 补充剂更高（P<0.01）。剂量和来源对鸡 RBC 抗原的效价没有影响。在第 150 天，用植物血凝素-P 进行的细胞介导免疫反应（迟发型超敏反应，DTH）和刀豆球蛋白 A 的体外淋巴细胞增殖反应，用 15ppm Zn 补充剂的羔羊比 BD 喂养的羔羊更高（P<0.05）。45ppm Zn 的补充对免疫反应没有积极影响。在 15ppm Zn 补充剂时，用 Zn 蛋白补充剂的 DTH 反应和针对 B.abortus 的抗体效价比用 ZnSO4 更高（P<0.05）。在 15ppm Zn 补充剂时，与 BD 日粮喂养的羔羊相比，脂质过氧化酶活性更低（P<0.01），而 RBC 超氧化物歧化酶和过氧化氢酶活性更高（P<0.01），在 75 天的喂养期。血清球蛋白浓度和碱性磷酸酶（ALP）活性（实验的第 75 天）在添加 Zn 的羔羊中更高。随着 Zn 补充量的增加，ALP 活性增加（P<0.01），当补充剂来自 Zn 蛋白时，比来自 ZnSO4 时更高。研究表明，当羔羊饲喂含有 29.28ppm Zn 的基础日粮时，15ppm 的锌补充量是获得更高免疫反应所必需的，而用 Zn 蛋白补充剂比用 ZnSO4 补充剂具有更高的抗氧化酶活性和免疫反应。
