Inaoka Takatoshi, Nakagawa Takayuki, Kikkawa Yayoi S, Tabata Yasuhiko, Ono Kazuya, Yoshida Mitsuhiro, Tsubouchi Hirohito, Ido Akio, Ito Juichi
Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Acta Otolaryngol. 2009 Apr;129(4):453-7. doi: 10.1080/00016480902725197.
Local application of hepatocyte growth factor using biodegradable gelatin hydrogels attenuates noise-induced hearing loss in guinea pigs.
To develop an inner ear drug delivery system using gelatin hydrogels that is capable of a sustained delivery of growth factors to the cochlea. We examined the efficacy of the local application of gelatin hydrogels containing hepatocyte growth factor (HGF) in protecting cochlear hair cells from noise-induced damage.
A piece of gelatin hydrogel previously immersed in either HGF or saline was placed on the round window membrane of a guinea pig 1 h after noise exposure (4 kHz octave band noise at 120 dB sound pressure level for 3 h). Auditory function was monitored using auditory brainstem responses (ABRs), and the loss of hair cells was evaluated quantitatively.
Local HGF treatment significantly reduced the noise exposure-caused ABR threshold shifts and the loss of outer hair cells in the basal portion of the cochleae.
使用可生物降解的明胶水凝胶局部应用肝细胞生长因子可减轻豚鼠噪声性听力损失。
开发一种使用明胶水凝胶的内耳给药系统，该系统能够将生长因子持续递送至耳蜗。我们研究了局部应用含肝细胞生长因子（HGF）的明胶水凝胶在保护耳蜗毛细胞免受噪声诱导损伤方面的效果。
在噪声暴露（120 dB声压级的4 kHz倍频程带噪声，持续3小时）1小时后，将一片预先浸泡在HGF或盐水中的明胶水凝胶置于豚鼠的圆窗膜上。使用听性脑干反应（ABR）监测听觉功能，并定量评估毛细胞的损失。
局部HGF治疗显著降低了噪声暴露引起的ABR阈值变化以及耳蜗基部外毛细胞的损失。
