通过牛颗粒细胞α-酰胺化酶将两栖动物水蛋白 2 异源转化为血管加压素。

Heterologue conversion of amphibian hydrin 2 into vasotocin through bovine granule alpha-amidating enzyme.

机构信息

Laboratory of Biological Chemistry, University of Paris VI, 96, Boulevard Raspail, 75006 Paris, France.

出版信息

J Neuroendocrinol. 1991 Feb 1;3(1):15-20. doi: 10.1111/j.1365-2826.1991.tb00233.x.

DOI:10.1111/j.1365-2826.1991.tb00233.x

PMID:19215441

Abstract

Abstract Hydrin 2 (vasotocinyl-Gly), found along with vasotocin in the neurohypophysis of frogs and toads but not of other vasotocin-bearers such as birds and reptiles, is believed to act on water permeability of frog skin, whereas vasotocin mainly fulfils the antidiuretic function on the kidney. In order to understand the peculiar regulation of provasotocin differential processing in amphibians, conversion of hydrin 2 into vasotocin has been attempted using bovine pituitary granule alpha-amidating enzyme. Generated vasotocin has pharmacological properties and Chromatographic behaviour in high-performance liquid chromatography identical to those of synthetic vasotocin. However, the low yield of conversion (5% to 10%) suggests that additional factor(s) might be involved in the physiological processing.

摘要

摘要蛙和蟾蜍神经垂体中发现的 Hydrin 2（血管加压素-Gly）与血管加压素一起存在，但在鸟类和爬行动物等其他血管加压素载体中不存在，据信它作用于青蛙皮肤的水通透性，而血管加压素主要在肾脏上发挥抗利尿作用。为了了解两栖动物 provasotocin 差异加工的特殊调节，使用牛垂体颗粒α-酰胺化酶尝试将 Hydrin 2 转化为血管加压素。生成的血管加压素有与合成血管加压素相同的药理学性质和高效液相色谱行为。然而，转化率低（5%至 10%）表明可能涉及其他因素（s）参与生理加工。