Omran Ghada A, Rutty Guy N, Jobling Mark A
East Midlands Forensic Pathology Unit, University of Leicester, Robert Kilpatrick Clinical Sciences Building, Leicester Royal Infirmary, Leicester LE2 7LX, UK.
Forensic Sci Int Genet. 2009 Mar;3(2):e39-44. doi: 10.1016/j.fsigen.2008.05.007. Epub 2008 Jul 2.
A sample of 265 unrelated individuals inhabiting five governorates in Upper (south) Egypt was collected with informed consent. The samples were amplified using the AmpFlSTR IdentifilerPCR Amplification Kit (containing 15 loci: D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA), and genotyped subsequent to capillary electrophoresis. Statistical analysis of the generated data indicated neither departure from expectation of Hardy-Weinberg Equilibrium (HWE) in most of the tested loci nor dependence of alleles between loci. All tested loci were polymorphic; the most discriminating is D18S51 while the least is TPOX. The combined power of exclusion was 0.99999868 and the combined match probability was 1.93x10(-18). The genetic diversity of the Upper Egyptians was compared with those of other populations at the local, regional and global levels.
在获得知情同意后，采集了居住在埃及上埃及（南部）五个省份的265名无血缘关系个体的样本。使用AmpFlSTR IdentifilerPCR扩增试剂盒（包含15个基因座：D8S1179、D21S11、D7S820、CSF1PO、D3S1358、TH01、D13S317、D16S539、D2S1338、D19S433、vWA、TPOX、D18S51、D5S818和FGA）对样本进行扩增，并在毛细管电泳后进行基因分型。对生成数据的统计分析表明，在大多数测试基因座中，既未偏离哈迪-温伯格平衡（HWE）预期，基因座之间的等位基因也不存在依赖性。所有测试基因座均具有多态性；鉴别力最强的是D18S51，最弱的是TPOX。联合排除概率为0.99999868，联合匹配概率为1.93×10（-18）。将上埃及人的遗传多样性与当地、区域和全球层面的其他人群进行了比较。
