Wang Ruo-jing, Yang Bin, Fu Mei-hong
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2008 Nov;33(22):2642-6.
To develop methods for qualitative and quantitative analyses of Flos Cartnami from three aspects, pigments, flavonoids and adenosine.
A method using HPLC coupled with electrochemical detector was developed to determine the content of hydroxysafflor yellow A and fingerprint of Flos Carthami. The chromatographic separation was performed on a Zorbax SB C18 column (4.6 mm x 250 mm, 5 microm) by gradient elution with phosphate buffer and acetonitrile at a flow-rate of 1.0 mL x min(-1), the column temperature was 35 degrees C, the reference electrode was ISAAC (in-situ silver/silver chloride), the working electrode was glassy carbon, the counter electrode was Pt, and the applied potential was + 800 mV. Concentration of adenosine was determined by HPLC-UV on an Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) with water-acetonitrile (95:5) as mobile phase, the flow rate was 1.0 mL x min(-1), the column temperature was 40 degrees C and the detection wavelength was 260 nm. The content of cartharmin was detected using a spectrophotometric method.
Twenty-one common chromatographic peaks were selected as characteristic peaks in the chromatogram of sample solution of Flos Cartnami. Seven peaks were identified as hydroxysafflor yellow A, 6-hydroxykaempferol-3-O-glucoside, rutin, quercetin-3-O-glucoside, kaempferol-3-O-rutinoside, quercetin, kaempferol. The contents of hydroxysafflor yellow A and adenosine were from 0.35% to 3.58% and from 0.03% per hundred to 0.49% per hundred, respectively.
The methods can be used to evaluate the quality of Flos Carthami.
从色素、黄酮类化合物和腺苷三个方面建立西红花的定性和定量分析方法。
建立了一种采用高效液相色谱-电化学检测器联用的方法来测定羟基红花黄色素A的含量和西红花的指纹图谱。色谱分离在Zorbax SB C18柱（4.6 mm×250 mm，5μm）上进行，以磷酸盐缓冲液和乙腈梯度洗脱，流速为1.0 mL·min⁻¹，柱温为35℃，参比电极为ISAAC（原位银/氯化银），工作电极为玻碳电极，对电极是铂电极，施加电位为+800 mV。腺苷的含量采用高效液相色谱-紫外检测法在Diamonsil C18柱（4.6 mm×250 mm，5μm）上测定，流动相为水-乙腈（95:5），流速为1.0 mL·min⁻¹，柱温为40℃，检测波长为260 nm。采用分光光度法检测红花苷的含量。
在西红花样品溶液的色谱图中选择了21个共有色谱峰作为特征峰。7个峰被鉴定为羟基红花黄色素A、6-羟基山柰酚-3-O-葡萄糖苷、芦丁、槲皮素-3-O-葡萄糖苷、山柰酚-3-O-芸香糖苷、槲皮素、山柰酚。羟基红花黄色素A和腺苷的含量分别为0.35%至3.58%和万分之三至万分之四十九。
这些方法可用于评价西红花的质量。
