[金荞麦根茎的高效液相色谱指纹图谱研究]

[Studies on HPLC-fingerprint of Rhizoma Fagopyri Dibotoryis].

作者信息

Yang Bin, Xin Min-tong, Lu Dong-mei, Qu Qiao-ling, Li Hua, Huang Lu-qi

机构信息

Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2007 Jun;32(12):1171-4.

PMID:17802879

Abstract

OBJECTIVE

To establish an HPLC fingerprint of Rhizoma fagopyri dibotoryis.

METHOD

The HPLC-electrochemical detection assay was used to establish the fingerprint of Rhizoma fagopyri dibotoryis. The sample was performed on a column of Diamonsil C18 (4.6 mm x 250 mm, 5 microm) which was eluted with methanol- 0.1 mol x L(-1) phosphate buffer (pH 2.5), the flow rate was 1.0 mL x min(-1), the column temperature was 35 degrees C, the reference electrode was ISAAC (in-situ silver/silver chloride), the work electrode was glassy carbon, the counter electrode was Pt platmun.

RESULT

The HPLC fingerprint profiles of 6 Rhizoma fagopyri dibotoryis contains 6 common chromatographic peaks, and gallic acid, protocatechuic acid, protocatechuic aldehyde and ( - ) -epicatechin were tested the samples. The contents of protocatechuic acid and protocatechuic aldehyde were from 0.004% to 0.05% and from 0.003% to 0.015%, respectively.

CONCLUSION

The method can be used to control the quality of Rhizoma fagopyri dibotoryis.

摘要

目的

建立金荞麦的高效液相色谱指纹图谱。

方法

采用高效液相色谱 - 电化学检测法建立金荞麦指纹图谱。样品在Diamonsil C18柱（4.6 mm×250 mm，5μm）上进行分析，以甲醇 - 0.1 mol·L⁻¹磷酸盐缓冲液（pH 2.5）为流动相，流速为1.0 mL·min⁻¹，柱温为35℃，参比电极为ISAAC（原位银/氯化银），工作电极为玻碳电极，对电极是铂电极。