Department of Biochemistry, Biology and Genetics, Marche Polytechnic University, Ancona, Italy.
Fertil Steril. 2010 Apr;93(6):1976-82. doi: 10.1016/j.fertnstert.2008.12.060. Epub 2009 Feb 12.
To investigate the role played by platelet nitric oxide (NO) metabolism in patients with spontaneous miscarriage (SM) and recurrent spontaneous miscarriage (RSM) compared with healthy pregnant women.
Retrospective case-control study.
Patients and controls in an academic research environment.
INTERVENTION(S): None.
PATIENT(S): Thirty singleton pregnant women who experienced SM, nine singleton pregnant women who presented with RSM, and 30 singleton healthy pregnant women matched for age, parity, and gestational age were enrolled.
MAIN OUTCOME MEASURE(S): NO levels and peroxynitrite (ONOO(-)) production; moreover, inducible NO synthase (iNOS), endothelial NO synthase (eNOS), and nitrotyrosine expression (N-Tyr) were observed in the same samples.
RESULT(S): A significant increase was shown in platelet NO and ONOO(-) levels and in iNOS and N-Tyr both in SM and in RSM pregnant women compared with controls.
CONCLUSION(S): The data herein reported imply that a modified NO pathway might play a key role in the physiological changes of advancing gestation but may also contribute to the pathophysiology of spontaneous miscarriage. Thus, any factors balancing NO metabolism might be useful in the treatment of miscarriage, thus reducing the substantial morbidity and associated mortality.
研究自发性流产（SM）和复发性自发性流产（RSM）患者血小板一氧化氮（NO）代谢的作用，并与健康孕妇进行比较。
回顾性病例对照研究。
学术研究环境中的患者和对照。
无。
招募了 30 名经历 SM 的单胎孕妇、9 名出现 RSM 的单胎孕妇和 30 名年龄、产次和孕龄匹配的单胎健康孕妇。
NO 水平和过氧亚硝酸盐（ONOO（-））产生；此外，还观察了相同样本中的诱导型一氧化氮合酶（iNOS）、内皮型一氧化氮合酶（eNOS）和硝基酪氨酸表达（N-Tyr）。
与对照组相比，SM 和 RSM 孕妇的血小板 NO 和 ONOO（-）水平以及 iNOS 和 N-Tyr 均显著增加。
本文报道的数据表明，改变的 NO 途径可能在妊娠进展的生理变化中起关键作用，但也可能导致自发性流产的病理生理变化。因此，平衡 NO 代谢的任何因素都可能有助于治疗流产，从而降低大量发病率和相关死亡率。
