Department of Biochemistry, Biology and Genetics, Marche Polytechnic University , via Tronto 10 - 60128 Ancona, Italy.
Platelets. 2012;23(1):26-35. doi: 10.3109/09537104.2011.589543. Epub 2011 Jul 25.
The aim of the study was to investigate platelet nitric oxide (NO) pathways in women with Gestational Hypertension (GH), Preeclampsia (PE) and Controls. Platelet NO(x) and peroxynitrite (ONOO(-)) levels, inducible (iNOS) and endothelial nitric oxide synthase (eNOS) and Nitrotyrosine expression (N-Tyr) in 30 women with GH, 30 with PE and 30 healthy pregnant controls, age, parity and gestational age-matched, were assessed. Platelet NO(x) and ONOO(-) levels were significantly higher in GH and PE vs. Controls, with higher levels in GH vs. PE. At the same way, iNOS and N-Tyr were significantly higher in GH and PE vs. Controls, with higher levels in GH vs. PE. Since GH expressed higher amount of NO metabolites and higher activation of iNOS compared to PE, we can hypothesize that the severity of hypertensive pathology is almost not related to only NO metabolism, this research confirmed that GH and PE are associated with marked changes in NO pathways; it is not easy to understand if they could be interpreted as causes or consequence of these pathologic states.
本研究旨在探讨妊娠期高血压（GH）、子痫前期（PE）妇女的血小板一氧化氮（NO）途径。评估了 30 例 GH 患者、30 例 PE 患者和 30 例年龄、产次和孕龄匹配的健康孕妇对照组的血小板 NO（x）和过氧亚硝酸盐（ONOO（-））水平、诱导型（iNOS）和内皮型一氧化氮合酶（eNOS）以及硝基酪氨酸表达（N-Tyr）。与对照组相比，GH 和 PE 患者的血小板 NO（x）和 ONOO（-）水平显著升高，且 GH 患者的水平高于 PE 患者。同样，GH 和 PE 患者的 iNOS 和 N-Tyr 水平也明显高于对照组，且 GH 患者的水平高于 PE 患者。由于 GH 表达的 NO 代谢物数量高于 PE，iNOS 的激活程度也高于 PE，因此我们可以假设高血压病理的严重程度几乎与仅 NO 代谢无关，本研究证实 GH 和 PE 与 NO 途径的明显变化相关；目前尚不清楚它们是否可以被解释为这些病理状态的原因或结果。
