Williams Lisa K, Jørgensen Frieda, Grogono-Thomas Rose, Humphrey Tom J
University of Bristol, Langford, UK.
Int J Food Microbiol. 2009 Mar 31;130(2):131-4. doi: 10.1016/j.ijfoodmicro.2009.01.018. Epub 2009 Jan 24.
Isolation of Campylobacter spp. using enrichment culture is time consuming and complex. Reducing the time taken to confirm the presence or absence of Campylobacter spp. would have many advantages for diagnostic, commercial and research applications. Rapid techniques such as real-time PCR can detect campylobacters from complex samples but blood in enrichment culture can inhibit the PCR reaction, if applied directly to enriched samples. The aim of this study was to investigate the effect of blood in enrichment culture on the isolation of campylobacters from chicken caeca, carcass rinses and bootsock (gauze sock walked through a broiler chicken house) samples using Bolton broth. The effect of incubation temperature (37 degrees C or 41.5 degrees C for 48 h, or 37 degrees C for 4 h then transfer to 41.5 degrees C for 44 h) and method of generating atmosphere (incubation of container in jar gassed with microaerobic atmosphere or incubation of container with small headspace and tightly screwed lid in an aerobic atmosphere) with and without blood on isolation from chicken carcass rinses and chicken faeces was also investigated. The presence of blood in enrichment culture did not improve the isolation of campylobacters from chicken faeces or bootsock samples but significantly improved recovery from chicken carcass rinse samples. There was no significant effect of the method used to generate incubation atmosphere. Isolation rates did also not depend significantly on whether broths were incubated at 37 or 41.5 degrees C for 24 or 48 h. Overall, the presence of blood in such media is not essential, although isolation can vary depending on sample type and enrichment method used.
使用增菌培养法分离弯曲杆菌属细菌既耗时又复杂。缩短确认弯曲杆菌属细菌存在与否所需的时间，对诊断、商业和研究应用都将具有诸多优势。实时荧光定量PCR等快速技术能够从复杂样本中检测出弯曲杆菌，但如果直接将富集培养物中的血液应用于富集样本，可能会抑制PCR反应。本研究的目的是调查富集培养物中的血液对使用博尔顿肉汤从鸡盲肠、胴体冲洗液和靴袜（在肉鸡舍中走过的纱布袜）样本中分离弯曲杆菌的影响。还研究了培养温度（37℃或41.5℃培养48小时，或37℃培养4小时后转移至41.5℃培养44小时）以及产生气体环境的方法（将容器置于用微需氧气体充气的罐中培养，或在有氧环境中将容器置于小顶空并拧紧盖子培养）对从鸡胴体冲洗液和鸡粪便中分离弯曲杆菌的影响，包括有无血液存在的情况。富集培养物中存在血液并不能提高从鸡粪便或靴袜样本中分离弯曲杆菌的效率，但能显著提高从鸡胴体冲洗液样本中的回收率。用于产生培养气体环境的方法没有显著影响。分离率也不显著取决于肉汤是在37℃还是41.5℃下培养24小时或48小时。总体而言，尽管分离情况可能因样本类型和所使用的富集方法而异，但此类培养基中血液的存在并非必不可少。
