Xiong Wenjun, Dabbouseh Noura M, Rebay Ilaria
Ben May Department for Cancer Research, University of Chicago, IL 60637, USA.
Dev Cell. 2009 Feb;16(2):271-9. doi: 10.1016/j.devcel.2008.12.005.
Eyes absent (Eya), named for its role in Drosophila eye development but broadly conserved in metazoa, possesses dual functions as a transcriptional coactivator and protein tyrosine phosphatase. Although Eya's transcriptional activity has been extensively characterized, the physiological requirements for its phosphatase activity remain obscure. In this study, we provide insight into Eya's participation in phosphotyrosine-mediated signaling networks by demonstrating cooperative interactions between Eya and the Abelson (Abl) tyrosine kinase during development of the Drosophila larval visual system. Mechanistically, Abl-mediated phosphorylation recruits Eya to the cytoplasm, where in vivo studies reveal a requirement for its phosphatase function. Thus, we propose a model in which, in addition to its role as a transcription factor, Eya functions as a cytoplasmic protein tyrosine phosphatase.
无眼蛋白（Eya），因其在果蝇眼睛发育中的作用而得名，但在多细胞动物中广泛保守，具有转录共激活因子和蛋白酪氨酸磷酸酶的双重功能。尽管Eya的转录活性已得到广泛表征，但其磷酸酶活性的生理需求仍不清楚。在本研究中，我们通过证明在果蝇幼虫视觉系统发育过程中Eya与阿贝尔森（Abl）酪氨酸激酶之间的协同相互作用，深入了解了Eya参与磷酸酪氨酸介导的信号网络。从机制上讲，Abl介导的磷酸化将Eya招募到细胞质中，体内研究表明其磷酸酶功能是必需的。因此，我们提出了一个模型，其中Eya除了作为转录因子发挥作用外，还作为细胞质蛋白酪氨酸磷酸酶发挥作用。
