Deatly A M, Spivack J G, Lavi E, O'Boyle D R, Fraser N W
Wistar Institute, Philadelphia, Pennsylvania 19104-4268.
J Virol. 1988 Mar;62(3):749-56. doi: 10.1128/JVI.62.3.749-756.1988.
Herpes simplex virus type 1 (HSV-1) DNA and RNA have been detected in peripheral nervous system (PNS) and central nervous system (CNS) tissues of latently infected mice. However, explant methods are successful in reactivating HSV-1 only from latently infected PNS tissues. In this report, latent herpesvirus infections in mouse PNS and CNS tissues were compared by in situ hybridization to determine whether the difference in reactivation was at the level of the virus or the host tissue. It was demonstrated that the HSV-1 transcripts present during latency in the mouse PNS and CNS originated from the same region of the genome, the repeats which bracket the long unique sequence. Therefore, the difference in reactivation with PNS and CNS tissues cannot be accounted for by differences in the extent of the HSV-1 genome transcribed during herpesvirus latency. Latent HSV-1 RNA was detected in the trigeminal ganglia (PNS) and the trigeminal system in the CNS from the mesencephalon to the spinal cord as well as other regions of the CNS not noted previously. Latent HSV-1 RNA was found predominantly in neurons but also in a small number of cells which could not be identified as neuronal cells. It is suggested that host differences in CNS and PNS tissues, rather than differences in latent virus transcription, may be important determinants in the HSV-1 reactivation process in explanted tissues.
在潜伏感染小鼠的外周神经系统（PNS）和中枢神经系统（CNS）组织中已检测到1型单纯疱疹病毒（HSV-1）的DNA和RNA。然而，外植体方法仅能成功地从潜伏感染的PNS组织中重新激活HSV-1。在本报告中，通过原位杂交比较了小鼠PNS和CNS组织中的潜伏性疱疹病毒感染，以确定重新激活的差异是在病毒水平还是宿主组织水平。结果表明，小鼠PNS和CNS潜伏期间存在的HSV-1转录本源自基因组的同一区域，即包围长独特序列的重复序列。因此，PNS和CNS组织重新激活的差异不能用疱疹病毒潜伏期间转录的HSV-1基因组范围的差异来解释。在三叉神经节（PNS）以及从中脑到脊髓的CNS中的三叉神经系统以及先前未提及的CNS其他区域中检测到潜伏性HSV-1 RNA。潜伏性HSV-1 RNA主要在神经元中发现，但也在少数无法鉴定为神经细胞的细胞中发现。有人提出，CNS和PNS组织中的宿主差异而非潜伏病毒转录的差异可能是外植组织中HSV-1重新激活过程的重要决定因素。
