Apps D K, Gleed C D
Biochem J. 1976 Nov;159(2):441-3. doi: 10.1042/bj1590441.
The interaction of pigeon liver NAD kinase with Cibacron Blue F3GA was investigated. By using steady-state rate measurements, spectrophotometric titration and chromatography of the enzyme on immobilized dye, it was shown that binding occurs at two nucleotide sites with different affinities, and also at a site distinct from the substrate-binding region.
研究了鸽肝NAD激酶与汽巴蓝F3GA的相互作用。通过稳态速率测量、分光光度滴定以及酶在固定化染料上的色谱分析,结果表明结合发生在两个具有不同亲和力的核苷酸位点,并且还发生在一个与底物结合区域不同的位点。