State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Plant Physiol. 2012 Feb;158(2):813-23. doi: 10.1104/pp.111.187666. Epub 2011 Dec 2.
MicroRNAs (miRNAs) and small interfering RNAs are important regulators of plant development and seed formation, yet their population and abundance in the oil crop Brassica napus are still not well understood, especially at different developmental stages and among cultivars with varied seed oil contents. Here, we systematically analyzed the small RNA expression profiles of Brassica napus seeds at early embryonic developmental stages in high-oil-content and low-oil-content B. napus cultivars, both cultured in two environments. A total of 50 conserved miRNAs and 9 new miRNAs were identified, together with some new miRNA targets. Expression analysis revealed some miRNAs with varied expression levels in different seed oil content cultivars or at different embryonic developmental stages. A large number of 23-nucleotide small RNAs with specific nucleotide composition preferences were also identified, which may present new classes of functional small RNAs.
microRNAs (miRNAs) 和 small interfering RNAs 是植物发育和种子形成的重要调控因子，然而，它们在油料作物油菜中的群体和丰度仍不清楚，尤其是在不同的发育阶段和种子含油量不同的品种之间。在这里，我们系统地分析了高油含量和低油含量油菜品种在两种环境下培养的早期胚胎发育阶段的油菜种子的小 RNA 表达谱。总共鉴定了 50 个保守的 miRNA 和 9 个新的 miRNA，以及一些新的 miRNA 靶标。表达分析显示，一些 miRNA 在不同种子含油量的品种或不同胚胎发育阶段的表达水平不同。还鉴定了大量具有特定核苷酸组成偏好的 23 核苷酸小 RNA，它们可能代表新的功能小 RNA 类别。
