Bhattacharjee Partha S, Neumann Donna M, Hill James M
Department of Ophthalmology, Louisiana State University Health Sciences Center, New Orleans, Lousiana, USA.
Curr Eye Res. 2009 Feb;34(2):99-102. doi: 10.1080/02713680802647662.
To compare the antiviral effect of a peptide derived from human apolipoprotein E (1% apoEdp) with 1% trifluorothymidine against HSV-1 thymidine kinase (TK)-positive (HSV-1 McKrae) and with 3% foscarnet against HSV-1 TK-negative (KOS background) in the rabbit eye model of acute HSV-1 epithelial keratitis.
Topical treatment began three days post-infection and continued for five days. Rabbits were treated 5 times per day with 1% apoEdp, 1% trifluorothymidine, 3% foscarnet, or PBS. Slit lamp examination was performed on a masked basis from post-infection days 3-10 to determine the severity of epithelial keratitis.
Topical treatment with 1% apoEdp was as effective as 1% trifluorothymidine against TK-positive and 3% foscarnet against TK-negative HSV-1 epithelial keratitis.
ApoEdp 1% has efficacy for the topical treatment of TK-positive and TK-negative ocular herpes in the rabbit eye model. Thus, apoEdp has the potential to be used for treatment of keratitis caused by TK mutants.
在单纯疱疹病毒1型（HSV-1）急性上皮性角膜炎的兔眼模型中，比较源自人载脂蛋白E的一种肽（1%载脂蛋白E衍生肽）与1%三氟胸腺嘧啶核苷对HSV-1胸苷激酶（TK）阳性（HSV-1 McKrae株）的抗病毒效果，以及与3%膦甲酸钠对HSV-1 TK阴性（KOS背景）的抗病毒效果。
感染后三天开始局部治疗，并持续五天。兔子每天用1%载脂蛋白E衍生肽、1%三氟胸腺嘧啶核苷、3%膦甲酸钠或磷酸盐缓冲液（PBS）治疗5次。在感染后第3至10天进行遮蔽式裂隙灯检查，以确定上皮性角膜炎的严重程度。
1%载脂蛋白E衍生肽局部治疗对TK阳性HSV-1上皮性角膜炎的效果与1%三氟胸腺嘧啶核苷相当，对TK阴性HSV-1上皮性角膜炎的效果与3%膦甲酸钠相当。
在兔眼模型中，1%载脂蛋白E衍生肽对TK阳性和TK阴性眼部疱疹的局部治疗有效。因此，载脂蛋白E衍生肽有潜力用于治疗由TK突变体引起的角膜炎。
