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由梭菌肽酶A消化和高压液相色谱法产生的人胶原蛋白“指纹图谱”

Human collagen 'fingerprints' produced by clostridopeptidase A digestion and high-pressure liquid chromatography.

作者信息

van Der Rest M, Cole W G, Glorieux F H

出版信息

Biochem J. 1977 Mar 1;161(3):527-34. doi: 10.1042/bj1610527.

DOI:10.1042/bj1610527
PMID:192198
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1164537/
Abstract

Samples (1-2mg) of purified human type I, II and III collagens and alpha1(I) and alpha2 chains were digested with clostridiopeptidase A and the released peptides analysed by ion-exchange high-pressure liquid chromatography. Specific 'fingerprints' were produced for each type of collagen. The reproducible nature of these 'fingerprints' and the reconstitution of the type I 'fingerprint' from the 'fingerprints' of the component alpha1(I) and alpha2 chains showed that the specificity of these 'fingerprints' was related to the primary structure of each type of collagen. In addition, some of the differences observed between the 'fingerprints' of the alpha1(I) and alpha2 chains of type I collagen were shown to be suitable for the quantitative analysis of these chains.

摘要

将纯化的人I型、II型和III型胶原蛋白以及α1(I)和α2链的样品（1 - 2毫克）用梭菌肽酶A消化，释放的肽通过离子交换高压液相色谱进行分析。每种胶原蛋白都产生了特定的“指纹图谱”。这些“指纹图谱”的可重复性以及由组成α1(I)和α2链的“指纹图谱”重构I型“指纹图谱”表明，这些“指纹图谱”的特异性与每种胶原蛋白的一级结构有关。此外，I型胶原蛋白α1(I)和α2链的“指纹图谱”之间观察到的一些差异被证明适用于这些链的定量分析。

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The collagen crosslinking in the hypophosphatemic male mouse.低磷血症雄性小鼠中的胶原蛋白交联
Calcif Tissue Int. 1981;33(1):77-9. doi: 10.1007/BF02409415.

本文引用的文献

1
A BIOCHEMICAL STUDY OF HUMAN SKIN COLLAGEN AND THE RELATION BETWEEN INTRA- AND INTERMOLECULAR CROSS-LINKING.人类皮肤胶原蛋白的生化研究以及分子内和分子间交联的关系
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Collagen heterogeneity. High resolution separation of native ( 1(I) 2 2 and ( 1(II) 3 and their component chains.胶原蛋白异质性。天然（1(I)22和(1(II)3及其组成链的高分辨率分离。
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Collagen polymorphism: characterization of molecules with the chain composition (alpha 1 (3)03 in human tissues.胶原蛋白多态性：人体组织中具有链组成（α1(3)03）的分子的特征
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Isolation and characterization of the cyanogen bromide peptides from the alpha 1(II) chain of bovine and human cartilage collagen.从牛和人软骨胶原蛋白α1(II)链中分离并鉴定溴化氰肽段
Biochemistry. 1973 Aug 14;12(17):3153-9. doi: 10.1021/bi00741a003.
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Comparative solubility of bone and skin collagen in primates including man.
J Med Primatol. 1972;1(1):58-67. doi: 10.1159/000460362.
6
(Alpha1(3))3 human skin collagen. Release by pepsin digestion and preponderance in fetal life.（α1(3)）3型人皮肤胶原蛋白。通过胃蛋白酶消化释放且在胎儿期占优势。
J Biol Chem. 1974 May 25;249(10):3225-31.
7
Human aorta collagens: evidence for three distinct species.人主动脉胶原蛋白：三种不同类型的证据。
Biochem Biophys Res Commun. 1974 Apr 8;57(3):717-25. doi: 10.1016/0006-291x(74)90605-6.
8
Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin.利用胃蛋白酶进行有限裂解和溶解对软骨胶原蛋白进行的结构研究。
Biochemistry. 1972 Dec 19;11(26):4903-9. doi: 10.1021/bi00776a005.
9
Lung collagen heterogeneity.肺胶原异质性。
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2828-32. doi: 10.1073/pnas.71.7.2828.
10
Precursors of collagen secreted by cultured human fibroblasts.培养的人成纤维细胞分泌的胶原蛋白前体。
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3655-9. doi: 10.1073/pnas.69.12.3655.