Seyer J M, Kang A H, Whitaker J N
Biochim Biophys Acta. 1977 Jun 24;492(2):415-25. doi: 10.1016/0005-2795(77)90093-9.
The normal chemical features of peripheral nerve collagens were determined on postmortem, histologically normal adult human femoral nerve. 1. Genetically distinct type I, [alpha1(I)2]alpha2, and type III, [alpha1(III)]3, were isolated by differential salt precipitation and the component subunit chains, alphal(I), alpha2 and alphal(III) were obtained by ion-exchange chromatography and gel filtration. 2. The molecular weight of alphal(I) and alpha2 of type I collagen was 95 000 and that for type III was 280 000. Reduction of type III with dithiothreitol yielded expected alpha1(III) chains of 95 000 molecular weight. 3. The amino acid composition of the three collagen chains, alpha1(I), alpha2, and alpha1(III), was the same as previously reported values for the corresponding chains from human skin except for slightly elevated hydroxylysine content. 4. Peripheral nerve collagen was found to contain 81% type I collagen and 19% type III. These results indicate that peripheral nerve collagen characteristics closely simulate that of human skin and differ from that of human aorta and other parenchymal organs. These data will permit a chemical analysis for possible abnormalities of peripheral nerve collagen in various neurogenic disorders.
在外死后组织学正常的成人股神经上测定了周围神经胶原的正常化学特征。1. 通过分级盐沉淀分离出基因上不同的I型（[α1(I)2]α2）和III型（[α1(III)]3）胶原，通过离子交换色谱法和凝胶过滤获得组成亚基链α1(I)、α2和α1(III)。2. I型胶原的α1(I)和α2的分子量为95000，III型胶原的分子量为280000。用二硫苏糖醇还原III型胶原可得到预期的分子量为95000的α1(III)链。3. 三种胶原链α1(I)、α2和α1(III)的氨基酸组成与先前报道的来自人皮肤的相应链的值相同，只是羟赖氨酸含量略有升高。4. 发现周围神经胶原含有81%的I型胶原和19%的III型胶原。这些结果表明，周围神经胶原特征与人类皮肤的特征非常相似，与人类主动脉和其他实质器官的特征不同。这些数据将有助于对各种神经源性疾病中周围神经胶原可能存在的异常进行化学分析。
