Tanganurat Winee, Quinquis Benoit, Leelawatcharamas Vichien, Bolotin Alexander
Department of Biotechnology, Kasetsart University, Bangkok, Thailand.
J Basic Microbiol. 2009 Aug;49(4):377-85. doi: 10.1002/jobm.200800185.
Ten Lactobacillus strains originally isolated from Thai fruits and vegetables fermentation were characterized by various phenotypic and genotypic methods. The phenotypic analysis using the method of carbohydrate fermentation patterns (API50CHL) revealed that the isolates belonged to the L. plantarum species. This was further confirmed by 16S rRNA gene sequencing. Multilocus sequence typing (MLST) revealed a strongly clonal population structure and a low genotypic diversity in this collection. However, the analyzed L. plantarum population demonstrated a higher level of diversification after API50CHL that reflects the role of available carbohydrate sources in bacterial evolution. Our results support the postulate that a combination of conventional biochemical and genotyping methods allows a thorough characterization and identification of isolates. We propose that genotypic characterization could be complemented by biochemical characterization to discriminate L. plantarum strains.
最初从泰国水果和蔬菜发酵物中分离出的10株乳酸杆菌菌株，通过各种表型和基因型方法进行了表征。使用碳水化合物发酵模式方法（API50CHL）进行的表型分析表明，这些分离株属于植物乳杆菌种。16S rRNA基因测序进一步证实了这一点。多位点序列分型（MLST）显示该菌群体结构高度克隆且基因型多样性较低。然而，经API50CHL分析后，所分析的植物乳杆菌群体表现出更高水平的多样化，这反映了可用碳水化合物源在细菌进化中的作用。我们的结果支持这样的假设，即传统生化方法和基因分型方法相结合能够对分离株进行全面表征和鉴定。我们建议，可以通过生化表征对基因型表征进行补充，以区分植物乳杆菌菌株。
