Laboratory of Applied Microbiology, Department of Microbiology, Faculty of Natural and Life Sciences, University Sétif 1 Ferhat Abbas, 19000 Sétif, Algeria.
Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Natural and life Sciences, University Sétif 1 Ferhat Abbas, 19000 Sétif, Algeria.
Biosci Microbiota Food Health. 2014;33(1):11-23. doi: 10.12938/bmfh.33.11. Epub 2014 Jan 30.
In the present work, five hundred and sixty-seven isolates of lactic acid bacteria were recovered from raw bee pollen grains. All isolates were screened for their antagonistic activity against both Gram-positive and Gram-negative pathogenic bacteria. Neutralized supernatants of 54 lactic acid bacteria (LAB) cultures from 216 active isolates inhibited the growth of indicator bacteria. They were phenotypically characterized, based on the fermentation of 39 carbohydrates. Using the simple matching coefficient and unweighted pair group algorithm with arithmetic averages (UPGMA), seven clusters with other two members were defined at the 79% similarity level. The following species were characterized: Lactobacillus plantarum, Lactobacillus fermentum, Lactococcus lactis, Pediococcus acidilactici, Pediococcus pentosaceus, and unidentified lactobacilli. Phenotypic characteristics of major and minor clusters were also identified. Partial sequencing of the 16S rRNA gene of representative isolates from each cluster was performed, and ten strains were assigned to seven species: Lactobacillus plantarum, Lactobacillus fermentum, Lactococcus lactis, Lactobacillus ingluviei, Pediococcus pentosaceus, Lactobacillus acidipiscis and Weissella cibaria. The molecular method used failed to determine the exact taxonomic status of BH0900 and AH3133.
在本工作中，从原蜂花粉粒中回收了 567 株乳酸菌分离物。所有分离物均针对革兰氏阳性和革兰氏阴性致病菌的拮抗活性进行了筛选。从 216 株活性分离物中，有 54 株乳酸菌（LAB）培养物的中和上清液抑制了指示菌的生长。基于 39 种碳水化合物的发酵，对其进行了表型特征分析。采用简单匹配系数和不带权的对组平均算法（UPGMA），在 79%的相似度水平上定义了七个具有其他两个成员的聚类。鉴定的以下菌种有：植物乳杆菌、发酵乳杆菌、乳球菌、戊糖片球菌、和未鉴定的乳杆菌。还鉴定了主要和次要聚类的表型特征。对每个聚类的代表性分离株的 16S rRNA 基因进行了部分测序，有 10 株菌株被分配到 7 个种：植物乳杆菌、发酵乳杆菌、乳球菌、乳杆菌 ingluviei、戊糖片球菌、嗜酸乳杆菌和希氏乳杆菌。使用的分子方法未能确定 BH0900 和 AH3133 的准确分类地位。
