Dorman C J
Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College Dublin, Dublin, UK.
Clin Microbiol Infect. 2009 Jan;15 Suppl 1:47-50. doi: 10.1111/j.1469-0691.2008.02684.x.
A powerful combination of single-gene studies and whole genome approaches has provided a wealth of information about the regulatory circuits used by bacteria to adapt to the environmental changes that are encountered during infection. The facultative intracellular pathogen Salmonella enterica will be used to illustrate how global regulators such as the nucleoid-associated proteins Fis and H-NS collaborate with fluctuations in the superhelicity of the DNA template to modify the gene expression profile of the bacterial cell outside and inside the host.
单基因研究与全基因组方法的强大结合，提供了大量关于细菌用于适应感染过程中所遇到的环境变化的调控回路的信息。兼性胞内病原体肠炎沙门氏菌将被用于阐明诸如类核相关蛋白Fis和H-NS等全局调控因子如何与DNA模板超螺旋度的波动协同作用，以改变宿主内外细菌细胞的基因表达谱。
