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与抗原加白细胞介素-5刺激下免疫球蛋白转录增加相关的新型蛋白质-DNA相互作用。

Novel protein-DNA interactions associated with increased immunoglobulin transcription in response to antigen plus interleukin-5.

作者信息

Webb C F, Das C, Eaton S, Calame K, Tucker P W

机构信息

Department of Immunobiology, Oklahoma Medical Research Foundation, Oklahoma City 73104.

出版信息

Mol Cell Biol. 1991 Oct;11(10):5197-205. doi: 10.1128/mcb.11.10.5197-5205.1991.

DOI:10.1128/mcb.11.10.5197-5205.1991
PMID:1922039
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC361554/
Abstract

Although much has been learned about basal levels of immunoglobulin (Ig) transcription, the regulatory effects of cytokines and antigen (Ag) upon Ig expression in lymphocytes have not been fully characterized. We previously reported that Ag plus interleukin-5 (IL-5) caused increased steady-state Ig mRNA levels in Ag-specific cell lines. In this study, we have identified a region between -250 and -125 bp 5' of the Ig transcription start site that is necessary for the induction of increased mu mRNA levels by Ag plus IL-5. Mobility shift and UV cross-linking studies indicated that IL-5 plus Ag induced increased protein binding to this region. Furthermore, this sequence was found to be closely related to another A + T-rich sequence at -525 bp 5' of the transcription start site. Both sequences exhibited similar B-cell-specific and inducible protein binding. Our data suggest that treatment with IL-5 plus Ag induces several DNA-binding proteins, some of which may participate in increasing Ig transcription above basal levels by binding to sequences 5' of the octamer motif.

摘要

尽管关于免疫球蛋白（Ig）转录的基础水平已了解很多，但细胞因子和抗原（Ag）对淋巴细胞中Ig表达的调节作用尚未完全阐明。我们先前报道，抗原加白细胞介素-5（IL-5）可使抗原特异性细胞系中的稳态Ig mRNA水平升高。在本研究中，我们确定了Ig转录起始位点5'端-250至-125 bp之间的一个区域，该区域对于抗原加IL-5诱导μ mRNA水平升高是必需的。凝胶迁移和紫外线交联研究表明，IL-5加抗原可诱导该区域的蛋白质结合增加。此外，发现该序列与转录起始位点5'端-525 bp处的另一个富含A + T的序列密切相关。两个序列均表现出相似的B细胞特异性和诱导性蛋白质结合。我们的数据表明，用IL-5加抗原处理可诱导几种DNA结合蛋白，其中一些可能通过与八聚体基序5'端的序列结合而参与将Ig转录提高到基础水平以上。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d050/361554/86cf566a9291/molcellb00034-0409-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d050/361554/626925d46814/molcellb00034-0405-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d050/361554/dc3a733b0111/molcellb00034-0405-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d050/361554/06f3b03670cf/molcellb00034-0406-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d050/361554/96386030bb8c/molcellb00034-0407-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d050/361554/1b86df0f00c4/molcellb00034-0408-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d050/361554/86cf566a9291/molcellb00034-0409-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d050/361554/626925d46814/molcellb00034-0405-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d050/361554/dc3a733b0111/molcellb00034-0405-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d050/361554/06f3b03670cf/molcellb00034-0406-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d050/361554/96386030bb8c/molcellb00034-0407-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d050/361554/1b86df0f00c4/molcellb00034-0408-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d050/361554/86cf566a9291/molcellb00034-0409-a.jpg

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