Looper M L, Edrington T S, Callaway T R, Rosenkrans C F
USDA-ARS, Dale Bumpers Small Farms Research Center, Booneville, AR, USA.
Lett Appl Microbiol. 2009 May;48(5):513-6. doi: 10.1111/j.1472-765X.2009.02563.x. Epub 2009 Feb 9.
To investigate the potential transfer of Escherichia coli O157:H7 and Salmonella from contaminated manure slurry into the tissue of tall fescue plants.
Tall fescue plants (n = 50) were fertilized with a manure slurry inoculated with E. coli O157:H7 and Salmonella. Soil was collected and tall fescue plants (n = 10 per day) harvested on day 1, 2, 4, 8, and 14 after manure slurry fertilization. Soil samples were positive for E. coli O157:H7 on all days and on day 1, 2, 8, and 14 for Salmonella. None of the plant tissue samples were positive for E. coli O157:H7 on day 1 or 2; however, 20%, 30% and 40% of plant tissue samples were positive for E. coli O157:H7 on day 4, 8, and 14, respectively.
It may be possible that E. coli O157:H7 can become transmitted and internalized into tall fescue plant tissue within 4 days after exposure to an E. coli O157:H7-contaminated manure slurry. Salmonella did not appear to be transferred to tall fescue plant tissue.
Faeces contaminated with E. coli O157:11H7 may be one means by which grazing ruminants spread bacterial pathogens to additional animals.
研究受污染的粪肥中大肠杆菌O157:H7和沙门氏菌向高羊茅植物组织的潜在转移情况。
用接种了大肠杆菌O157:H7和沙门氏菌的粪肥对50株高羊茅植物施肥。在粪肥施肥后的第1、2、4、8和14天采集土壤，并收获高羊茅植物（每天10株）。所有天数的土壤样本中大肠杆菌O157:H7均呈阳性，沙门氏菌在第1、2、8和14天呈阳性。在第1天或第2天，没有植物组织样本中大肠杆菌O157:H7呈阳性；然而，在第4、8和14天，分别有20%、30%和40%的植物组织样本中大肠杆菌O157:H7呈阳性。
在接触受大肠杆菌O157:H7污染的粪肥后4天内，大肠杆菌O157:H7有可能传播并内化到高羊茅植物组织中。沙门氏菌似乎没有转移到高羊茅植物组织中。
受大肠杆菌O157:11H7污染的粪便可能是放牧反刍动物将细菌病原体传播给其他动物的一种方式。
