Department of Microbial Ecology, Centre for Ecological and Evolutionary Studies, University of Groningen, Kerklaan 30, 9750 AA Haren, The Netherlands.
J Environ Manage. 2011 Mar;92(3):780-7. doi: 10.1016/j.jenvman.2010.10.031. Epub 2010 Oct 28.
The influence of aerobic and anaerobic conditions on the survival of the enteropathogens Escherichia coli O157:H7 and Salmonella serovar Typhimurium was investigated in microcosms with broth, cattle manure or slurry. These substrates were inoculated with a green fluorescent protein transformed strain of the enteropathogens at 10(7) cells g(-1) dry weight. Survival data was fitted to the Weibull model. The survival curves in aerobic conditions generally showed a concave curvature, while the curvature was convex in anaerobic conditions. The estimated survival times showed that E. coli O157:H7 survived significantly longer under anaerobic than under aerobic conditions. Survival ranged from approximately. 2 weeks for aerobic manure and slurry to more than six months for anaerobic manure at 16 °C. On average, in 56.3% of the samplings, the number of recovered E. coli O157:H7 cells by anaerobic incubation of Petri plates was significantly (p < 0.05) higher in comparison with aerobic incubation. Survival of Salmonella serovar Typhimurium was not different between aerobic and anaerobic storage of LB broth or manure as well as between aerobic and anaerobic incubation of Petri dishes. The importance of changes in microbial community and chemical composition of manure and slurry was distinguished for the survival of E. coli O157:H7 in different oxygen conditions.
研究了在含有肉汤、牛粪便或粪浆的微环境中，有氧和无氧条件对肠病原体大肠杆菌 O157:H7 和沙门氏菌 Typhimurium 存活的影响。这些基质用 10(7)个细胞 g(-1)干重的肠病原体绿色荧光蛋白转化株接种。生存数据拟合 Weibull 模型。有氧条件下的生存曲线通常呈凹形曲率，而无氧条件下的曲率呈凸形。估计的生存时间表明，大肠杆菌 O157:H7 在厌氧条件下比有氧条件下存活时间显著延长。在 16°C 下，有氧粪便和粪浆的存活时间约为 2 周，而厌氧粪便的存活时间超过 6 个月。平均而言，在 56.3%的采样中，通过厌氧培养 Petri 平板回收的大肠杆菌 O157:H7 细胞数量明显（p < 0.05）高于有氧培养。在 LB 肉汤或粪便的有氧和无氧储存以及 Petri 盘的有氧和无氧培养中，沙门氏菌 Typhimurium 的存活没有差异。对于大肠杆菌 O157:H7 在不同氧气条件下的存活，粪便和粪浆中微生物群落和化学成分的变化的重要性得到了区分。
