Yin Min, Yuan Yuan, Liu Changsheng, Wang Jing
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
Biomaterials. 2009 May;30(14):2764-73. doi: 10.1016/j.biomaterials.2009.01.039. Epub 2009 Feb 14.
In this study, to improve the attachment, growth and adhesion of endothelial cells (ECs) and thus accelerate the re-endothelialization of stents, a synthesized mussel adhesive polypeptide mimics containing dihydroxyphenylalanine and L-lysine (MAPDL) was immobilized onto 316L stainless steel (316LSS) with polyethylene glycol (PEG) molecule as spacer arm by using cold plasma-induced grafting technique. To immobilize MAPDL effectively, ethylene vinyl acetate (EVA) was first coated onto 316LSS. Different molecular weights of PEG and grafting times were tested to obtain the optimal cell bioactivity. XPS and water contact angles measurement indicated the successful immobilization of MAPDL. In vitro cell culture results showed that compared with the control of 316LSS, the attachment, adhesion and growth of cells on the MAPDL-coated EVA surface, in particular with PEG as spacer arm, were significantly enhanced, and a confluent endothelial cells layer was formed after a 2-day culture. A platelet adhesion experiment revealed that the platelet adhesion was also reduced on the MAPDL-coated EVA surface. The in vitro inflammatory assessment showed that the MAPDL coating inhibited the TNF-alpha and IL-1beta release from monocyte cells, indicative of good anti-inflammation property. Therefore, it is concluded that the MAPDL coating developed here appeared to be a promising strategy for rapid re-endothelialization of intravascular stent devices.
在本研究中，为了改善内皮细胞（ECs）的附着、生长和黏附，从而加速支架的再内皮化，采用冷等离子体诱导接枝技术，以聚乙二醇（PEG）分子为间隔臂，将一种合成的含二羟基苯丙氨酸和L-赖氨酸的贻贝黏附多肽模拟物（MAPDL）固定在316L不锈钢（316LSS）上。为了有效固定MAPDL，首先将乙烯-醋酸乙烯酯（EVA）涂覆在316LSS上。测试了不同分子量的PEG和接枝时间以获得最佳的细胞生物活性。X射线光电子能谱（XPS）和水接触角测量表明MAPDL已成功固定。体外细胞培养结果表明，与316LSS对照组相比，在MAPDL涂覆的EVA表面，特别是以PEG为间隔臂的表面上，细胞的附着、黏附和生长显著增强，培养2天后形成了融合的内皮细胞层。血小板黏附实验表明，在MAPDL涂覆的EVA表面血小板黏附也减少。体外炎症评估表明，MAPDL涂层抑制了单核细胞释放肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β），表明具有良好的抗炎特性。因此，得出结论，本文开发的MAPDL涂层似乎是一种用于血管内支架装置快速再内皮化的有前景的策略。
