Key Lab. of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu, 610031, China.
Adv Healthc Mater. 2012 Sep;1(5):548-59. doi: 10.1002/adhm.201200073. Epub 2012 Jul 25.
Polydopamine (PDAM), a mussel adhesive protein inspired coating that can be easily deposited onto a wide range of metallic, inorganic, and organic materials, gains interest also in the field of biomaterials. In this work, PDAM is applied as coating on 316L stainless steel (SS) stents and the response of cells of the blood vessel wall, human umbilical vein endothelial cell (HUVEC), and human umbilical artery smooth muscle cell (HUASMC) as predictors for re-endothelialization is tested. It is found that the PDAM-modified surface significantly enhances HUVEC adhesion, proliferation, and migration, release of nitric oxide (NO), and secretion of prostaglandin I(2) (PGI(2) ). Additionally, the PDAM-modified surface shows a remarkable ability to decrease the adhesion and proliferation of HUASMCs. As a blood-contacting material, the PDAM tends to improve the hemocompatibility compared with the substrate 316L SS. It is noteworthy that the PDAM coating shows good resistance to the deformation behavior of compression and expansion of a stent. These data suggest the potential of PDAM as a blood-contacting material for the application in vascular stents or grafts.
聚多巴胺（PDAM）是一种受贻贝启发的黏附蛋白涂层，可以很容易地沉积在各种金属、无机和有机材料上，它在生物材料领域也引起了关注。在这项工作中，PDAM 被用作 316L 不锈钢（SS）支架的涂层，血管壁细胞、人脐静脉内皮细胞（HUVEC）和人脐动脉平滑肌细胞（HUASMC）的反应被用作再内皮化的预测因子进行测试。结果发现，PDAM 修饰表面显著增强了 HUVEC 的黏附、增殖和迁移能力，释放一氧化氮（NO）和前列环素 I（2）（PGI（2））。此外，PDAM 修饰表面显示出显著降低 HUASMC 黏附和增殖的能力。作为一种与血液接触的材料，与基底 316L SS 相比，PDAM 倾向于改善血液相容性。值得注意的是，PDAM 涂层具有良好的抗压和扩张变形行为的阻力。这些数据表明 PDAM 作为一种与血液接触的材料，在血管支架或移植物中的应用具有潜力。
