Casabianca Leah B, Kallgren Joye B, Natarajan Jayakumar K, Alumasa John N, Roepe Paul D, Wolf Christian, de Dios Angel C
Department of Chemistry, Georgetown University, NW Washington, DC 20057, United States.
J Inorg Biochem. 2009 May;103(5):745-8. doi: 10.1016/j.jinorgbio.2009.01.013. Epub 2009 Jan 27.
Proton nuclear magnetic resonance relaxation times were measured for the protons of micelles formed by the detergents sodium dodecyl sulfate, dodecyltrimethyl ammonium bromide, and polyethylene glycol sorbitan monolaureate in the presence of ferriprotoporphyrin IX and the antimalarial drugs chloroquine, 7-chloro-4-quinolyl 4-N,N-diethylaminobutyl sulfide, and primaquine. Diffusion coefficients were extracted from pulsed gradient NMR experiments to evaluate the degree of association of these drugs with the detergent micelles. Results indicate that at low or neutral pH when the quinolyl N is protonated, chloroquine does not associate with neutral or cationic detergent micelles. For this reason, chloroquine's interaction with heme perturbs the partitioning of heme between the aqueous medium and detergent micelles.
在高铁原卟啉IX以及抗疟药物氯喹、7-氯-4-喹啉基4-N，N-二乙氨基丁基硫醚和伯氨喹存在的情况下，测量了由去污剂十二烷基硫酸钠、十二烷基三甲基溴化铵和聚乙二醇山梨醇单月桂酸酯形成的胶束中质子的质子核磁共振弛豫时间。从脉冲梯度核磁共振实验中提取扩散系数，以评估这些药物与去污剂胶束的缔合程度。结果表明，在低pH或中性pH下，当喹啉基N质子化时，氯喹不与中性或阳离子去污剂胶束缔合。因此，氯喹与血红素的相互作用扰乱了血红素在水相介质和去污剂胶束之间的分配。
