State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai 200237, China.
Appl Biochem Biotechnol. 2010 Mar;160(3):812-21. doi: 10.1007/s12010-009-8536-9. Epub 2009 Feb 18.
Brevibacillus brevis HOB1 was isolated from the formation water of an oil field and found to produce lipopeptides. The separation of lipopeptides was successfully achieved by reversed-phase high-performance liquid chromatography (HPLC) leading to nine separated peaks. The chemical structures of these lipopeptides were studied by means of electrospray ionization mass spectrometry (ESI-MS), gas chromatography-mass spectrometry (GC/MS), HPLC and electrospray ionization tandem mass spectrometry (ESI-MS/MS). As the results, all the lipopeptides had peptide parts with the same amino acid composition of Asp, Glu, Val, and Leu in the molar ratio 1:1:1:4, while the lipid part was composed of C(13)-C(15) beta-hydroxy fatty acids. As the sequence of fraction 1 was determined to be N-Glu-Leu-Leu-Val-Asp-Leu-Leu-C, the same as surfactin, they were proposed to be surfactin isoforms. Fraction 4 (C(15) surfactin) exhibited a good surface activity of 26.8 mN/m with CMC of 9 x 10(-6 )M. Surfactin is a powerful biosurfactant possessing biological activities. As far as we know, Br. brevis is a new surfactin-producing species.
短小芽孢杆菌 HOB1 从油田地层水中分离得到，能够产生脂肽。通过反相高效液相色谱（HPLC）成功地分离出 9 个分离峰。通过电喷雾电离质谱（ESI-MS）、气相色谱-质谱（GC/MS）、HPLC 和电喷雾串联质谱（ESI-MS/MS）研究了这些脂肽的化学结构。结果表明，所有脂肽的肽部分具有相同的氨基酸组成，即天冬氨酸、谷氨酸、缬氨酸和亮氨酸，摩尔比为 1:1:1:4，而脂部分由 C(13)-C(15)β-羟基脂肪酸组成。由于 1 馏分的序列被确定为 N-Glu-Leu-Leu-Val-Asp-Leu-Leu-C，与表面活性剂相同，因此它们被提议为表面活性剂异构体。4 馏分（C(15)表面活性剂）表现出良好的表面活性，表面张力为 26.8 mN/m，CMC 为 9×10(-6)M。表面活性剂是一种具有生物活性的强力生物表面活性剂。据我们所知，短小芽孢杆菌是一种新的产生表面活性剂的物种。
