Department of Environmental Engineering, Gebze Institute of Technology, 41400 Gebze, Turkey.
Appl Biochem Biotechnol. 2010 Mar;160(3):856-67. doi: 10.1007/s12010-009-8534-y. Epub 2009 Feb 18.
Polyvinylferrocene (PVF) was used as a mediator for the fabrication of a horseradish peroxidase (HRP)-modified electrode to detect phenol derivatives via a composite polymeric matrix of conducting polypyrrole (PPy). Through an electropolymerization process, enzyme HRP was entrapped with PPy in a three-electrode system onto a glassy carbon electrode previously covered with PVF, resulting in a composite polymeric matrix. Steady-state amperometric measurements were performed at -200 mV vs. Ag/AgCl in aqueous phosphate buffer containing NaCl 0.1 M (pH 6.8) in the presence of hydrogen peroxide. The response of the HRP-modified PVF electrode was investigated for various phenol derivatives, which were 4-chlorophenol, phenol, catechol, hydroquinone, 2-aminophenol, pyrogallol, m-cresol, and 4-methoxyphenol. Analytical parameters for the fabricated PVF electrode were obtained from the calibration curves. The highest sensitivity was obtained from the calibration of 4-chlorophenol as 29.91 nA/microM. The lowest detection limit was found to be 0.22 microM (S/N = 3) for catechol, and the highest detection limit was found to be 0.79 microM (S/N = 3) for 4-methoxyphenol among the tested derivatives. The biosensor can reach 95% of steady-state current in about 5 min. The electrode is stable for 2 months at 4 degrees Celsius.
聚二茂铁（PVF）被用作介体制备辣根过氧化物酶（HRP）修饰电极，通过导电聚吡咯（PPy）的复合聚合物基质来检测酚衍生物。通过电聚合过程，酶 HRP 与 PPy 在三电极系统中被包埋在先前用 PVF 覆盖的玻碳电极上，形成复合聚合物基质。在含有 0.1 M NaCl 的磷酸盐缓冲液（pH 6.8）中，在过氧化氢存在下，在 -200 mV 相对于 Ag/AgCl 进行稳态安培测量。研究了 HRP 修饰的 PVF 电极对各种酚衍生物的响应，包括 4-氯苯酚、苯酚、儿茶酚、对苯二酚、2-氨基酚、焦儿茶酚、间甲酚和 4-甲氧基苯酚。从校准曲线获得了用于制备的 PVF 电极的分析参数。从 4-氯苯酚的校准中获得了最高的灵敏度，为 29.91 nA/μM。对于儿茶酚，检测限最低为 0.22 μM（S/N = 3），对于测试的衍生物中的 4-甲氧基苯酚，检测限最高为 0.79 μM（S/N = 3）。生物传感器可以在大约 5 分钟内达到稳态电流的 95%。电极在 4°C 下稳定保存 2 个月。
