Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242-1109, USA.
Gene Ther. 2009 May;16(5):581-8. doi: 10.1038/gt.2009.16. Epub 2009 Feb 19.
In the past 2 years, new gene-targeting approaches using adeno-associated virus and designer zinc-finger nucleases have been successfully applied to the production of genetically modified ferrets, pigs, mice and zebrafish. Gene targeting using these tools has been combined with somatic cell nuclear transfer and germ cell transplantation to generate gene-targeted animal models. These new technical advances, which do not require the generation of embryonic stem cell-derived chimeras, will greatly accelerate the production of non-mouse animal models for biomedical research.
在过去的 2 年中，使用腺相关病毒和设计的锌指核酸酶的新基因靶向方法已成功应用于转基因雪貂、猪、鼠和斑马鱼的生产。使用这些工具的基因靶向已与体细胞核转移和生殖细胞移植相结合，以产生基因靶向动物模型。这些不需要生成胚胎干细胞衍生嵌合体的新技术进步将极大地加速用于生物医学研究的非小鼠动物模型的生产。
