Borisova N A, Sapronova A Y, Proshlyakova E V, Ugrumov M V
Laboratory of Hormonal Regulations, U.S.S.R. Academy of Sciences, Moscow.
Neuroscience. 1991;43(1):223-9. doi: 10.1016/0306-4522(91)90429-r.
The development of the hypothalamic catecholaminergic system during ontogenesis in rats has been studied with glyoxylic acid histofluorescent method in vivo and with isotopic biochemical technique in vitro. It has been demonstrated that at the 15th fetal day the catecholaminergic system was functionally inactive at least in its ability for the uptake and K(+)-stimulated release of catecholamines. Since the 16th fetal day, hypothalamic neuronal elements gained an ability for synthesis of catecholamines, their specific uptake and K(+)-evoked release. Over the subsequent two days, the intensity of the fluorescent intraneuronal product rose considerably showing the increase of either synthesis or accumulation of catecholamines. Simultaneously, the values of the uptake and K(+)-stimulated release of the exogenous radioactively-labelled dopamine increased significantly. The intensity of the fluorescence of the hypothalamic neuronal elements dropped from 20th fetal until the ninth postnatal day, whereas the specific uptake doubled over the same period reaching its adult level. By the 21st postnatal day the reaccumulation of the fluorescent product occurred.
采用体内乙醛酸组织荧光法和体外同位素生化技术，对大鼠个体发育过程中下丘脑儿茶酚胺能系统的发育进行了研究。结果表明，在胚胎第15天，儿茶酚胺能系统至少在摄取和钾离子刺激的儿茶酚胺释放能力方面功能不活跃。从胚胎第16天开始，下丘脑神经元成分获得了合成儿茶酚胺、特异性摄取和钾离子诱发释放儿茶酚胺的能力。在随后的两天里，神经元内荧光产物的强度显著增加，表明儿茶酚胺的合成或积累增加。同时，外源性放射性标记多巴胺的摄取和钾离子刺激释放的值显著增加。下丘脑神经元成分的荧光强度从胚胎第20天到出生后第9天下降，而在同一时期特异性摄取增加了一倍，达到成年水平。到出生后第21天，荧光产物再次积累。
