Ugrumov M V, Tixier-Vidal A, Taxi J, Thibault J, Mitskevich M S
Institute of Developmental Biology, U.S.S.R. Academy of Sciences, Moscow.
Neuroscience. 1989;29(1):157-66. doi: 10.1016/0306-4522(89)90339-4.
The innervation of the hypothalamus and septal region by catecholaminergic fibers was studied in rats from the 12th fetal day until the 9th postnatal day. Catecholaminergic fibers were visualized with preembedding immunocytochemistry using antibodies to tyrosine hydroxylase. An intensification of diaminobenzidine product with silver and gold was additionally applied to increase the sensitivity and resolution power of the routine immunocytochemical technique. It has been demonstrated that, from the 13th fetal day, the hypothalamus and the septal region receive catecholaminergic fibers either belonging to the hypothalamic neurons or coming with the medial forebrain bundle from the outside of the hypothalamus. As the development of the hypothalamus proceeds, these fibers form the extensive networks within some neurosecretory centers either containing (the zona incerta, periventricular nucleus, etc.) or almost lacking (suprachiasmatic and paraventricular nuclei) the catecholaminergic neurons. In the former case, they terminate on the processes or perikarya of catecholaminergic neurons, while in the latter case their varicosities surround the immunonegative presumptive neurons in a basket-like manner. Moreover, from the 18th fetal day catecholaminergic fibers penetrate between the ependymal cells towards the 3rd ventricle and the primary capillary plexus of the hypophysial portal circulation, apparently providing the release of catecholamines to the cerebrospinal fluid and portal blood, respectively. The data obtained in this study are considered as the morphological basis for the involvement of the hypothalamic catecholamines in neuroendocrine regulations during ontogenesis.
对从胚胎第12天至出生后第9天的大鼠，研究了儿茶酚胺能纤维对下丘脑和隔区的神经支配。使用酪氨酸羟化酶抗体，通过包埋前免疫细胞化学使儿茶酚胺能纤维可视化。另外采用银和金对二氨基联苯胺产物进行强化，以提高常规免疫细胞化学技术的灵敏度和分辨能力。已证实，从胚胎第13天起，下丘脑和隔区接受儿茶酚胺能纤维，这些纤维要么属于下丘脑神经元，要么从下丘脑外部随内侧前脑束而来。随着下丘脑的发育，这些纤维在一些神经分泌中心内形成广泛的网络，这些中心要么含有（未定带、室周核等）要么几乎缺乏（视交叉上核和室旁核）儿茶酚胺能神经元。在前一种情况下，它们终止于儿茶酚胺能神经元的突起或胞体上，而在后一种情况下，它们的膨体以篮状方式围绕免疫阴性的假定神经元。此外，从胚胎第18天起，儿茶酚胺能纤维穿入室管膜细胞之间，朝向第三脑室和垂体门脉循环的初级毛细血管丛，显然分别向脑脊液和门脉血释放儿茶酚胺。本研究获得的数据被视为下丘脑儿茶酚胺在个体发育过程中参与神经内分泌调节的形态学基础。
