Ugrumov M V, Proshlyakova E V, Sapronova A
Institute of Developmental Biology, U.S.S.R. Academy of Sciences, Moscow.
Neuroscience. 1989;32(1):127-31. doi: 10.1016/0306-4522(89)90112-7.
The development of the hypothalamic 5-hydroxytryptamine system has been evaluated in vitro according to [3H]5-hydroxytryptamine uptake and release in fetuses (16-20th fetal day), neonates (9th postnatal day) and adults (45th day of life). At the 16th fetal day the hypothalamic neural elements were characterized by specific uptake of 5-hydroxytryptamine and its spontaneous release; the next day K+-stimulated Ca2+-dependent release appeared. By the 18th fetal day, the 5-hydroxytryptamine uptake doubled and was retained at this level both in older fetuses and in postnatal rats. The K+-stimulated release of [3H]5-hydroxytryptamine increased considerably during the perinatal period, reaching an adult level by the 9th postnatal day. These data indicate the sprouting of 5-hydroxytryptamine fibers to the hypothalamus and the maturation of their membrane mechanisms for the bidirectional transport of 5-hydroxytryptamine early during ontogenesis-to a significant extent before the 18th fetal day.
根据胎儿（胎儿期第16 - 20天）、新生儿（出生后第9天）和成年大鼠（出生后第45天）下丘脑5-羟色胺系统的[3H]5-羟色胺摄取和释放情况，对其发育进行了体外评估。在胎儿期第16天，下丘脑神经元的特征是对5-羟色胺有特异性摄取及其自发释放；第二天出现了钾离子刺激的钙离子依赖性释放。到胎儿期第18天，5-羟色胺摄取量翻倍，并在较大胎儿和出生后大鼠中维持在这一水平。在围产期，钾离子刺激的[3H]5-羟色胺释放显著增加，到出生后第9天达到成年水平。这些数据表明，5-羟色胺纤维在下丘脑发芽，并且其5-羟色胺双向转运的膜机制在个体发育早期（在胎儿期第18天之前的很大程度上）就已成熟。
