Reuter Gábor, Fodor Domonka, Forgách Petra, Kátai Andrea, Szucs György
Gastroenteralis Vírusok Nemzeti Referencia Laboratóriuma, ANTSZ Dél-dunántúli Regionális Intézete Regionális Virológiai Laboratórium Pécs Szabadság u. 7. 7623.
Orv Hetil. 2009 Mar 1;150(9):415-21. doi: 10.1556/OH.2009.28561.
Hepatitis E virus (HEV) is one of a common cause of acute, fecally transmitted hepatitis in developing countries. Identification of HEV in indigenous human infection and in domestic pig raises the possibility that HEV infection is also a zoonosis.
AIM/METHODS: Molecular detection and epidemiology of HEV in humans with acute hepatitis and in domestic (pig, cattle) and wild (boar and roe-deer) animals by ELISA and RT-PCR in Hungary.
Between 2001 and 2006, a total of 116 (9.6%) human sera were positive by HEV IgM ELISA and 13 (24.5%) of 53 samples were also confirmed by RT-PCR and sequencing. Forty-two, 11 and 9 samples were RT-PCR-positive from swine (feces: 22.7%; liver: 30.8%), roe-deer (liver: 34.4%) and wild boar (liver: 12.2%), respectively. Except for an imported infection caused by genotype 1, 19 sequences (human: 12, swine: 4, roe-deer: 1, wild boar: 2) belong to genotype 3 HEV. Genetically identical strains were detected in human and roe-deer and in 2 other human clusters.
HEV is an endemic agent in Hungary. Consumption of raw or undercooked meat-products is one of the possible sources of the indigenous HEV infections. Cross-species infection with genotype 3 HEV involves a food-borne transmission route in Hungary.
戊型肝炎病毒（HEV）是发展中国家急性粪口传播型肝炎的常见病因之一。在本土人类感染病例及家猪中发现HEV，提示HEV感染也可能是人畜共患病。
目的/方法：通过酶联免疫吸附测定（ELISA）和逆转录聚合酶链反应（RT-PCR）对匈牙利急性肝炎患者、家（猪、牛）畜及野（野猪和狍）兽中的HEV进行分子检测和流行病学研究。
2001年至2006年期间，共有116份（9.6%）人类血清经HEV IgM ELISA检测呈阳性，53份样本中的13份（24.5%）也经RT-PCR及测序得以确认。猪（粪便：22.7%；肝脏：30.8%）、狍（肝脏：34.4%）和野猪（肝脏：12.2%）的样本中分别有42份、11份和9份经RT-PCR检测呈阳性。除1例由1型基因型引起的输入性感染外，19个序列（人类：12个，猪：4个，狍：1个，野猪：2个）属于3型基因型HEV。在人类和狍以及另外2个人类集群中检测到基因相同的毒株。
HEV在匈牙利呈地方性流行。食用生的或未煮熟的肉制品是本土HEV感染的可能来源之一。在匈牙利，3型基因型HEV的跨物种感染涉及食源性传播途径。
