Kia Eshrat Beigom
School of Public Health and Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran.
Southeast Asian J Trop Med Public Health. 2003;34 Suppl 2:108-9.
Echinococcus multilocularis, the small fox tapeworm, has an extensive geographical range in the northern hemisphere where foxes and small rodents represent natural hosts. The larval stage of this parasite, alveolar echinococcosis (AE), is an emerging zoonosis of increasing importance. It is a serious human illness which is often misdiagnosed as hepatic cancer. If not identified at an early stage of parasite development it can lead to the death of patients. Histological examination of biopsies is one of the classical methods of diagnosis. In this study, in order to gain unequivocal histopathological diagnosis of AE, the immunoperoxidase staining technique was performed on routinely processed histological sections of an experimentally infected gerbil, using rabbit anti-E. multilocularis protoscolex IgG labelled with horseradish peroxidase. Demonstration of AE antigen was achieved by dark brown stain of cyst membranes against a blue background of the host liver cells stained with hematoxylin.
多房棘球绦虫，即小型狐狸绦虫，在北半球具有广泛的地理分布范围，狐狸和小型啮齿动物是其天然宿主。这种寄生虫的幼虫阶段，即泡型包虫病（AE），是一种日益重要的新发人畜共患病。它是一种严重的人类疾病，常被误诊为肝癌。如果在寄生虫发育的早期阶段未被识别，可能会导致患者死亡。活检组织的组织学检查是经典的诊断方法之一。在本研究中，为了获得明确的AE组织病理学诊断，使用辣根过氧化物酶标记的兔抗多房棘球绦虫原头节IgG，对实验感染沙鼠的常规处理组织切片进行免疫过氧化物酶染色技术。通过苏木精染色的宿主肝细胞蓝色背景衬托下，囊肿膜呈深棕色染色来显示AE抗原。
