Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Bioorganic Chemistry & Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Biosens Bioelectron. 2009 Nov 15;25(3):615-22. doi: 10.1016/j.bios.2009.01.027. Epub 2009 Jan 31.
Surface imprinting is an effective approach to improve the template transfer efficiency in applications of molecularly imprinted polymers as biosensors and separation materials. In this paper, we tried to fabricate a surface imprinted hydrogel over silica microspheres for selective recognition of bovine serum albumin by covalent immobilization of a water-soluble UV sensitive initiator onto the surface of silica beads. The polymerization was initiated by UV radiation with N-[3-(dimethylamino)propyl]methacrylamide and N-isopropylacrylamide as the functional monomer and assistant monomer, respectively, and a thin coat of stimuli-responsive hydrogel yielded over the silica gels. The surface imprinted hydrogels exhibited specific affinity toward the template protein with an association constant (K(a)) of 2.2 x 10(5)L mol(-1) and a maximum binding capacity (Q(max)) of 27.3 mgg(-1) in Tris-HCl buffer (pH 7.0). The rebinding and desorption kinetics of the surface imprinted hydrogels were determined and proven to be extremely fast (about 1 min compared to 3h for the previously prepared bulk imprinted hydrogel). Besides, the hydrogel-silica core-shell particles inherit both the stimuli-responsive property of the hydrogel and the good mechanical strength of the silica beads based on the on-line evaluation with high-performance liquid chromatography. The above comprehensive merits of the obtained surface imprinted hydrogel suggest the presented approach an attractive and broadly applicable way of developing biosensors and high-performance protein separation materials.
表面印迹是一种有效的方法，可以提高分子印迹聚合物作为生物传感器和分离材料的模板传递效率。在本文中，我们尝试通过将水溶性 UV 敏感引发剂共价固定在硅胶珠表面，在硅胶微球上制备用于选择性识别牛血清白蛋白的表面印迹水凝胶。聚合通过 UV 辐射引发，分别使用 N-[3-(二甲氨基)丙基]甲基丙烯酰胺和 N-异丙基丙烯酰胺作为功能单体和辅助单体，在硅胶上生成一层薄的响应性水凝胶。表面印迹水凝胶对模板蛋白表现出特异性亲和力，在 Tris-HCl 缓冲液（pH 7.0）中的缔合常数 (K(a)) 为 2.2 x 10(5)L mol(-1)，最大结合容量 (Q(max)) 为 27.3 mgg(-1)。表面印迹水凝胶的再结合和解吸动力学被确定，并被证明非常快（与先前制备的本体印迹水凝胶相比，约 1 分钟）。此外，基于高效液相色谱的在线评估，水凝胶-硅胶核壳粒子继承了水凝胶的响应性和硅胶珠的良好机械强度。所得到的表面印迹水凝胶的上述综合优点表明，该方法是开发生物传感器和高性能蛋白质分离材料的一种有吸引力和广泛适用的方法。
