van Eijk Hans M H, Bloemen Johanne G, Dejong Cornelis H C
Maastricht University Medical Centre, Department of Surgery, NUTRIM School for Nutrition, Toxicology & Metabolism, P.O. Box 616, NL-6200 MD Maastricht, The Netherlands.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Mar 15;877(8-9):719-24. doi: 10.1016/j.jchromb.2009.01.039. Epub 2009 Feb 5.
A new liquid chromatography-mass spectrometry method is described to determine concentrations of the short chain fatty acids acetic acid, propionic acid and butyric acid (SCFAs) in human blood plasma. The method is based on reversed phase chromatography followed by post-column neutralization of the mobile phase with ammonia and a consecutive measurement of the SCFAs ammonia adducts using negative electro spray ionization. Sample preparation involved simple organic acid deproteinization, resulting in 100% recovery. SCFAs eluted baseline separated within a 25 min run cycle. A linear response was obtained in the range between 0 and 250 micromol/l (R(2) ranged from 0.997 to 0.9999). The limit of detection ranged from 0.05 micromol/l for propionic and butyric acid and 0.1 micromol/l for acetic acid. The method was tested by analyzing plasma of arterial blood, from portal vein and hepatic vein blood from patients undergoing a pylorus-preserving pancreaticoduodenectomy. As expected, the highest SCFA concentrations were found in portal plasma, hepatic vein levels were in between, while arterial concentrations were lowest. This newly developed method is suitable to determine SCFA concentrations in human plasma samples.
本文描述了一种新的液相色谱 - 质谱法，用于测定人血浆中短链脂肪酸乙酸、丙酸和丁酸（SCFAs）的浓度。该方法基于反相色谱，随后用氨对流动相进行柱后中和，并使用负电喷雾电离连续测量SCFAs氨加合物。样品制备包括简单的有机酸脱蛋白，回收率达100%。SCFAs在25分钟的运行周期内基线分离洗脱。在0至250微摩尔/升的范围内获得线性响应（R²范围为0.997至0.9999）。丙酸和丁酸的检测限为0.05微摩尔/升，乙酸的检测限为0.1微摩尔/升。通过分析接受保留幽门胰十二指肠切除术患者的动脉血、门静脉血和肝静脉血的血浆对该方法进行了测试。正如预期的那样，门静脉血浆中SCFA浓度最高，肝静脉水平介于两者之间，而动脉浓度最低。这种新开发的方法适用于测定人血浆样品中的SCFA浓度。
