Nishino Kunihiko, Nikaido Eiji, Yamaguchi Akihito
Department of Microbiology and Infectious Diseases, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567-0047, Japan.
Biochim Biophys Acta. 2009 May;1794(5):834-43. doi: 10.1016/j.bbapap.2009.02.002. Epub 2009 Feb 20.
Multidrug efflux is an obstacle to the successful treatment of infectious diseases, and it is mediated by multidrug efflux pumps that recognize and export a broad spectrum of chemically dissimilar toxic compounds. Many bacterial genome sequences have been determined, allowing us to identify drug efflux genes encoded in the bacterial genome. Here, we present an approach to identifying drug efflux genes and their regulatory networks in Escherichia coli and Salmonella. Multidrug efflux pumps are often regulated by environmental signals and they are required for bacterial virulence in addition to multidrug resistance. It is now understood that these efflux pumps also have physiological roles. In this article, we investigate the physiological roles of drug efflux pumps in virulence. Because multidrug efflux pumps have roles in bacterial drug resistance and virulence, we propose that drug efflux pumps have greater clinical relevance than previously considered.
多药外排是成功治疗传染病的一个障碍，它由多药外排泵介导，这些泵识别并输出多种化学性质不同的有毒化合物。许多细菌的基因组序列已被确定，这使我们能够识别细菌基因组中编码的药物外排基因。在此，我们提出一种在大肠杆菌和沙门氏菌中识别药物外排基因及其调控网络的方法。多药外排泵通常受环境信号调控，除了多药耐药性外，它们也是细菌毒力所必需的。现在人们认识到这些外排泵也具有生理作用。在本文中，我们研究药物外排泵在毒力方面的生理作用。由于多药外排泵在细菌耐药性和毒力方面发挥作用，我们认为药物外排泵具有比以前认为的更大的临床相关性。
