Baldwin Carson D, Howe Gerald B, Sampath Ranga, Blyn Larry B, Matthews Heather, Harpin Vanessa, Hall Thomas A, Drader Jared J, Hofstadler Steve A, Eshoo Mark W, Rudnick Karl, Studarus Karen, Moore David, Abbott Sharon, Janda J Michael, Whitehouse Chris A
US Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA.
Diagn Microbiol Infect Dis. 2009 Apr;63(4):403-8. doi: 10.1016/j.diagmicrobio.2008.12.012. Epub 2009 Feb 18.
Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. Here, 142/156 (91%) isolates were correctly identified to the genus level and 115/156 (74%) were correctly identified to the species level. Only 9% were misidentified. This study shows that multilocus PCR/ESI-MS has the potential to be a useful technique for identifying a broad range of bacteria.
聚合酶链反应电喷雾电离质谱法（PCR/ESI-MS）被用于测试其准确鉴定一组156种不同细菌分离株的能力，这些分离株大多是人类和/或动物病原体。在此研究中，156株中有142株（91%）被正确鉴定到属水平，156株中有115株（74%）被正确鉴定到种水平。只有9%被错误鉴定。这项研究表明，多位点PCR/ESI-MS有潜力成为一种用于鉴定多种细菌的有用技术。
