Ueoka Reiko, Ito Akihiro, Izumikawa Miho, Maeda Satoko, Takagi Motoki, Shin-ya Kazuo, Yoshida Minoru, van Soest Rob W M, Matsunaga Shigeki
Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Toxicon. 2009 May;53(6):680-4. doi: 10.1016/j.toxicon.2009.02.008.
Azaspiracid-2 was isolated from a marine sponge Echinoclathria sp. collected off Amami-Oshima as the predominant cytotoxic constituent. A combination of HPLC using ODS, GS320, and Phenylhexyl stationary phases permitted the purification without using acid or inorganic additives in the mobile phase. Azaspiracid-2 exhibited potent cytotoxicity against P388 cells with an IC50 value of 0.72 ng/mL and caused S phase arrest on the cell cycle.
azaspiracid-2是从大隅群岛附近采集的一种海洋海绵Echinoclathria sp.中分离出来的主要细胞毒性成分。使用ODS、GS320和苯基己基固定相的高效液相色谱法相结合，无需在流动相中使用酸或无机添加剂即可实现纯化。azaspiracid-2对P388细胞表现出强大的细胞毒性，IC50值为0.72 ng/mL，并导致细胞周期的S期停滞。
