Zhang Wenliang, Yu Lu, Yang Jian, Wang Lingling, Peng Junping, Jin Qi
State Key Laboratory for Molecular Virology and Genetic Engineering, Beijing 100176, China.
Appl Microbiol Biotechnol. 2009 Apr;82(6):1123-30. doi: 10.1007/s00253-009-1908-9. Epub 2009 Feb 24.
Trichophyton rubrum is a good model for the study of human pathogenic filamentous fungi. The antifungal agent terbinafine (TRB) shows specific activity against T. rubrum. To identify the transcriptional profiles of response to TRB in T. rubrum, a cDNA microarray was constructed from the expressed sequence tags of different phase cDNA libraries and transcriptional profiles of the response to TRB were determined. Exposure to TRB decreased the transcription of some genes involved in the ergosterol biosynthesis pathway, including ERG2, ERG4, ERG24, and ERG25, and induced the expression of some genes involved in lipid metabolism such as ERG10, ERG13, and INO1. TRB affected transcription of multidrug-resistance genes and some genes encoding ribosomal proteins.
红色毛癣菌是研究人类致病丝状真菌的良好模型。抗真菌药物特比萘芬（TRB）对红色毛癣菌具有特异性活性。为了鉴定红色毛癣菌对TRB反应的转录谱，利用不同阶段cDNA文库的表达序列标签构建了cDNA微阵列，并确定了对TRB反应的转录谱。暴露于TRB会降低参与麦角固醇生物合成途径的一些基因的转录，包括ERG2、ERG4、ERG24和ERG25，并诱导参与脂质代谢的一些基因的表达，如ERG10、ERG13和INO1。TRB影响多药耐药基因和一些编码核糖体蛋白的基因的转录。
