Sorg Joseph A, Dineen Sean S
Department of Molecular Biology and Microbiology, Tufts University, Boston, Massachusetts, USA.
Curr Protoc Microbiol. 2009 Feb;Chapter 9:Unit9A.1. doi: 10.1002/9780471729259.mc09a01s12.
Clostridium difficile is a Gram-positive, spore-forming, anaerobe and is the leading cause of antibiotic-associated diarrhea, pseudomembranous colitis, and toxic megacolon. Essential to the lifestyle of C. difficile is the ability to form a metabolically dormant spore, germinate, and grow out upon appropriate signals and elicit disease with the secretion of two toxins. To aid in the study of this organism, this unit describes the growth and maintenance of C. difficile. Included are methods to isolate C. difficile from environmental samples, grow in laboratory medium, and produce and purify spores.
艰难梭菌是一种革兰氏阳性、产芽孢的厌氧菌，是抗生素相关性腹泻、假膜性结肠炎和中毒性巨结肠的主要病因。艰难梭菌生活方式的关键在于能够形成代谢休眠的芽孢，在适当信号的作用下萌发并生长，通过分泌两种毒素引发疾病。为了辅助对这种微生物的研究，本单元描述了艰难梭菌的培养和保存方法。其中包括从环境样本中分离艰难梭菌、在实验室培养基中培养、以及产生和纯化芽孢的方法。
