Goswami Supriya, Vig Komal, Singh Dileep K
Department of Zoology, University of Delhi, Delhi 110007, India.
Chemosphere. 2009 May;75(7):883-8. doi: 10.1016/j.chemosphere.2009.01.057. Epub 2009 Feb 23.
The biodegradation of endosulfan and the metabolites formed were studied using fungi both in broth culture as well as in soil microcosm. Fungal strains were isolated from soil and grown in broth Czapek-dox medium. The strain which utilized endosulfan and showed maximum growth was selected for detailed studies. Maximum degrading capability in shake flask culture was shown by Aspergillus sydoni which degraded 95% of endosulfan alpha and 97% of endosulfan beta in 18 d of incubation. Soil microcosm study was also carried out using this strain in six different treatments. Endosulfan sulfate was the main metabolite formed along with small quantity of endosulfan ether and endosulfan lactone both in broth culture and soil microcosm. This isolated fungal strain will be a potential source for endosulfan degrading enzymes and can be used for bioremediation at the contaminated sites.
利用真菌在液体培养以及土壤微观系统中研究了硫丹及其形成的代谢产物的生物降解。从土壤中分离出真菌菌株，并在查氏培养基中进行培养。选择利用硫丹且生长最佳的菌株进行详细研究。在摇瓶培养中，西氏曲霉表现出最大降解能力，在18天的培养期内，它降解了95%的α-硫丹和97%的β-硫丹。还使用该菌株在六种不同处理下进行了土壤微观系统研究。在液体培养和土壤微观系统中，硫酸硫丹都是主要形成的代谢产物，同时还伴有少量的硫丹醚和硫丹内酯。这种分离出的真菌菌株将是硫丹降解酶的潜在来源，可用于受污染场地的生物修复。
