Department of Wood Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia,
World J Microbiol Biotechnol. 2013 Dec;29(12):2239-47. doi: 10.1007/s11274-013-1389-y. Epub 2013 Jun 5.
The ability of two white-rot fungi (Trametes versicolor and Pleurotus ostreatus) and one brown-rot fungus (Gloeophyllum trabeum) to degrade two organochlorine insecticides, lindane and endosulfan, in liquid cultures was studied and dead fungal biomass was examined for adsorption of both insecticides from liquid medium. Lindane and endosulfan were also treated with fungal laccase and bacterial protein CotA, which has laccase activities. The amount of degraded lindane and endosulfan increased with their exposure period in the liquid cultures of both examined white-rot fungi. Endosulfan was transformed to endosulfan sulphate by T. versicolor and P. ostreatus. A small amount of endosulfan ether was also detected and its origin was examined. Degradation of lindane and endosulfan by a brown rot G. trabeum did not occur. Mycelial biomasses of all examined fungi have been found to adsorb lindane and endosulfan and adsorption onto fungal biomass should therefore be considered as a possible mechanism of pollutant removal when fungal degradation potentials are studied. Bacterial protein CotA performed more efficient degradation of lindane and endosulfan than fungal laccase and has shown potential for bioremediation of organic pollutants.
两种白腐真菌（糙皮侧耳和香菇）和一种褐腐真菌（栓菌）在液体培养中降解两种有机氯杀虫剂林丹和硫丹的能力进行了研究，并检查了死真菌生物量对两种杀虫剂从液体培养基中的吸附作用。林丹和硫丹也用真菌漆酶和具有漆酶活性的细菌蛋白 CotA 进行了处理。在两种白腐真菌的液体培养中，林丹和硫丹的暴露时间越长，降解的林丹和硫丹的量就越多。硫丹被糙皮侧耳和香菇转化为硫丹硫酸盐。还检测到少量的硫丹醚，并对其来源进行了检查。褐腐菌栓菌未降解林丹和硫丹。所有被检测真菌的菌丝体生物量都被发现可以吸附林丹和硫丹，因此在研究真菌降解潜力时，应将吸附到真菌生物量上视为污染物去除的一种可能机制。细菌蛋白 CotA 对林丹和硫丹的降解效率高于真菌漆酶，具有生物修复有机污染物的潜力。
