Goswami Supriya, Singh Dileep K
Department of Zoology, University of Delhi, Delhi, India.
Biodegradation. 2009 Apr;20(2):199-207. doi: 10.1007/s10532-008-9213-3. Epub 2008 Aug 15.
Bacterial strains were isolated from endosulfan treated soil to study the microbial degradation of this pesticide in broth medium and soil microcosm. The isolates were grown in minimal medium and screened for endosulfan degradation. The strain, which utilized endosulfan and showed maximum growth, was selected for detail studies. Maximum degrading capability in shake flask culture was shown by Bordetella sp. B9 which degraded 80% of alpha endosulfan and 86% of beta endosulfan in 18 days. Soil microcosm study was also carried out using this strain in six different treatments. Endosulfan ether and endosulfan lactone were the main metabolites in broth culture, while in soil microcosm endosulfan sulfate was also found along with endosulfan ether and endosulfan lactone. This bacterial strain has a potential to be used for bioremediation of the contaminated sites.
从经硫丹处理的土壤中分离出细菌菌株，以研究该农药在肉汤培养基和土壤微观世界中的微生物降解情况。将分离物在基本培养基中培养，并筛选其对硫丹的降解能力。选择利用硫丹并表现出最大生长量的菌株进行详细研究。在摇瓶培养中，博德特氏菌属B9菌株表现出最大降解能力，在18天内降解了80%的α-硫丹和86%的β-硫丹。还使用该菌株在六种不同处理下进行了土壤微观世界研究。硫丹醚和硫丹内酯是肉汤培养中的主要代谢产物，而在土壤微观世界中，除了硫丹醚和硫丹内酯外，还发现了硫丹硫酸盐。这种细菌菌株具有用于污染场地生物修复的潜力。
