用于鉴定DNA中dI:dG错配碱基对结构的核磁共振研究。
NMR studies for identification of dI:dG mismatch base-pairing structure in DNA.
作者信息
Oda Y, Uesugi S, Ikehara M, Kawase Y, Ohtsuka E
机构信息
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
出版信息
Nucleic Acids Res. 1991 Oct 11;19(19):5263-7. doi: 10.1093/nar/19.19.5263.
Abstract
One- and two-dimensional NMR experiments have been undertaken to investigate deoxyinosine:deoxyguanosine (dI:dG) base pairing in a self-complementary dodecadeoxyribonucleotide, d(C1-G2-C3-I4-A5-A6-T7-T8-G9-G10-G11-G12) (designated IG-12), duplex. The NMR data indicate formation of a dI(syn):dG(anti) base pair in a B-DNA helix. This unusual base pairing results in altered NOE patterns between the base protons (H8 and H2) of the I4 residue and the sugar protons of its own and the 5'-flanking C3 residues. The dI(syn):dG(anti) base pair is accommodated in the B-DNA duplex with only a subtle distortion of the local conformation. Identification of the dI:dG base pairing in this study confirms that a hypoxanthine base can form hydrogen-bonded base pairs with all of the four normal bases, C, A, T, and G, in DNA.
摘要
已开展一维和二维核磁共振实验，以研究一种自我互补的十二聚脱氧核糖核苷酸d（C1-G2-C3-I4-A5-A6-T7-T8-G9-G10-G11-G12）（命名为IG-12）双链体中的脱氧次黄苷：脱氧鸟苷（dI：dG）碱基对。核磁共振数据表明在B-DNA螺旋中形成了dI（顺式）：dG（反式）碱基对。这种不寻常的碱基对导致I4残基的碱基质子（H8和H2）与其自身以及5'-侧翼C3残基的糖质子之间的核Overhauser效应（NOE）模式发生改变。dI（顺式）：dG（反式）碱基对存在于B-DNA双链体中，只是局部构象有细微扭曲。本研究中对dI：dG碱基对的鉴定证实，次黄嘌呤碱基可与DNA中的所有四种正常碱基C、A、T和G形成氢键结合的碱基对。
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