水溶液中r(CGCGCG)的构象分析：通过二维核Overhauser效应光谱研究的A型双螺旋构象
Conformational analysis of r(CGCGCG) in aqueous solution: an A-type double helical conformation studied by two-dimensional nuclear Overhauser effect spectroscopy.
作者信息
Westerink H P, van der Marel G A, van Boom J H, Haasnoot C A
出版信息
Nucleic Acids Res. 1984 May 25;12(10):4323-38. doi: 10.1093/nar/12.10.4323.
Abstract
The conformation of the hexanucleoside pentaphosphate r( CGCGCG ) in aqueous solution was studied by circular dichroism, 1H- and 31P-NMR spectroscopy. The base-, H1'- and H2'-proton resonances were assigned by means of 2D-NOE spectroscopy. The base- and H1'-proton chemical shifts were studied as a function of temperature. Proton-proton distances are computed in A- and A'-RNA as well as in A-, B- and Z-DNA. A qualitative interpretation of the observed 2D-NOE intensities shows that r( CGCGCG ) adopts a regular A-type double helical conformation under our experimental conditions. The CD- and 31P-NMR experiments described in this paper are in agreement with this structure both under low- and high-salt conditions.
摘要
通过圆二色性、1H-和31P-核磁共振光谱研究了六核苷五磷酸r（CGCGCG）在水溶液中的构象。借助二维核Overhauser效应光谱确定了碱基、H1'-和H2'-质子共振。研究了碱基和H1'-质子化学位移随温度的变化。计算了A-和A'-RNA以及A-、B-和Z-DNA中的质子-质子距离。对观察到的二维核Overhauser效应强度的定性解释表明，在我们的实验条件下，r（CGCGCG）采用规则的A型双螺旋构象。本文所述的圆二色性和31P-核磁共振实验在低盐和高盐条件下均与该结构一致。
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